CAJ | 학술논문

目的研究母鼠孕前慢性应激对成年雄性子代行为学和脑内单胺类神经递质的影响.方法 16只成年雌性SD大鼠随机分为正常对照组和慢性不可预见性应激组(CUS),CUS组大鼠每天接受一种应激,共21d,正常对照组不接受任何应激.应激结束后10 d,2组大鼠均与同种SD雄性大鼠合笼,以成年2月龄雄性子代作为研究对象.用糖水消耗实验测量快感缺失,Morris水迷宫检测空间记忆能力,高效液相色谱法测量海马、下丘脑和前额皮质单胺递质的含量.结果糖水消耗实验示正常对照组子代的糖水消耗量和糖水消耗百分比均显著高于CUS组子代[糖水消耗量:(10.23±4.12)g,(6.48±3.19)g;糖水消耗百分比:(85.43±20.15)%,(60.98±24.65)%](P＜0.05).Morris水迷宫检测示2组的逃避潜伏期无差异,但空间探索测试中正常对照组子代[(4.17±2.29)次]穿越平台的次数明显多CUS组子代[(1.64±1.69)次](P＜0.05).CUS组子代[(500.17±80.94)ng/g脑组织]下丘脑5-羟色胺的含量低于正常对照组子代[(569.63±50.91)ng/g脑组织](P＜0.05),而CUS组子代[(2315.01±1397.12)ng/g脑组织]海马去甲肾上腺素的含量却要高于正常对照组子代[(907.56±207.27)ng/g脑组织](P＜0.05).结论母鼠孕前慢性应激或抑郁会造成成年雄性子代的快感缺失和空间记忆力下降,以及脑内单胺类神经递质异常.
목적 연구모서잉전만성응격대성년웅성자대행위학화뇌내단알류신경체질적영향.방법 16지성년자성SD대서수궤분위정상대조조화만성불가예견성응격조(CUS),CUS조대서매천접수일충응격,공21d,정상대조조불접수임하응격.응격결속후10 d,2조대서균여동충SD웅성대서합롱,이성년2월령웅성자대작위연구대상.용당수소모실험측량쾌감결실,Morris수미궁검측공간기억능력,고효액상색보법측량해마、하구뇌화전액피질단알체질적함량.결과 당수소모실험시정상대조조자대적당수소모량화당수소모백분비균현저고우CUS조자대[당수소모량:(10.23±4.12)g,(6.48±3.19)g;당수소모백분비:(85.43±20.15)%,(60.98±24.65)%](P＜0.05).Morris수미궁검측시2조적도피잠복기무차이,단공간탐색측시중정상대조조자대[(4.17±2.29)차]천월평태적차수명현다CUS조자대[(1.64±1.69)차](P＜0.05).CUS조자대[(500.17±80.94)ng/g뇌조직]하구뇌5-간색알적함량저우정상대조조자대[(569.63±50.91)ng/g뇌조직](P＜0.05),이CUS조자대[(2315.01±1397.12)ng/g뇌조직]해마거갑신상선소적함량각요고우정상대조조자대[(907.56±207.27)ng/g뇌조직](P＜0.05).결론 모서잉전만성응격혹억욱회조성성년웅성자대적쾌감결실화공간기억력하강,이급뇌내단알류신경체질이상.
Objective To examine the effects of maternal rats exposed to chronic unpredictable stress before pregnancy on the behaviors and brain monamine of their adult male offspring.Methods Sixteen SD rats were divided into chronic unpredictable stress (CUS) group and controls.CUS rats were exposed to 21 days chronic unpredictable stressors ,and the controls were stress-free.Ten days after the last stressor, all the female rats were caged with sexually experienced males of the same strain.Then we performed the following experiments on the two months male progeny, sucrose consumption measuring anhedonia, Morris water maze measuring cognitive function and high performance liquid chromatography detecting the contents of monoamine.Results The sucrose consumption showed that both sucrose intake and sucrose consumption percentage of the control progeny were higher than those of the CUS progeny ( sucrose consumption: ( 10.23 ± 4.12 ) g vs ( 6.48 ± 3.19 ) g; sucrose consumption percentage: ( 85.43 ± 20.15 ) % vs (60.98 ± 24.65 ) % ) (P ＜ 0.05 ).The number of times crossing the removed hidden platform in the CUS progeny ( 1.64 ± 1.69) was significantly fewer than that in the control progeny (4.17±2.29 ) in Morris water maze (P ＜ 0.05 ).The contents of serotonin in the hypothalamus of CUS progeny ( ( 500.17 ± 80.94 ) ng/g tissue) was lower than that of the control progeny ( ( 569.63 ± 50.91 ) ng/g tissue) (P ＜0.05) ,while the norepinephrine in the hippocampus of CUS progeny( (2315.01 ± 1397.12) ng/g tissue) was higher than that of the control progeny( (907.56 ± 207.27) ng/g tissue) (P＜0.05) by high performance liquid chromatography.Conclusions Depression or stressful events before pregnancy of dams result in anhedonia, decreased spatial memory and abnormalities in brain monoamine of their adult male progeny.