中国临床营养杂志
中國臨床營養雜誌
중국림상영양잡지
CHINESE JOURNAL OF CLINICAL NUTRITION
2009年
1期
49-52
,共4页
二十二碳六烯酸%胰腺肿瘤%细胞凋亡%环氧合酶2
二十二碳六烯痠%胰腺腫瘤%細胞凋亡%環氧閤酶2
이십이탄륙희산%이선종류%세포조망%배양합매2
Docosahexaenic acid%Pancreatic cancer%Cell apoptosis%Cyclooxygenase-2
目的 探讨二十二碳六烯酸(DHA)对人胰腺癌细胞株Patu8988和SW1990生长的作用.方法 采用MTF法检测DHA作用后Patu8988和SW1990细胞的增殖,流式细胞术检测DHA作用后Patu8988和SW1990细胞的凋亡、细胞周期和肿瘤相关蛋白环氧合酶2(COX-2)的表达量.结果 DHA作用人胰腺癌细胞株24、48、72 h后,细胞的增殖受到明显抑制(P＜0.01),同时DHA能诱导细胞凋亡,随着作用时间延长和作用剂量增加,效果越明显.50μg/ml DHA作用24 h后,胰腺癌细胞的COX-2表达量下降(P＜0.05).结论 DHA能有效地抑制胰腺癌细胞增殖,同时诱导细胞凋亡,可能与COX-2在胰腺癌细胞中的表达下凋有关.
目的 探討二十二碳六烯痠(DHA)對人胰腺癌細胞株Patu8988和SW1990生長的作用.方法 採用MTF法檢測DHA作用後Patu8988和SW1990細胞的增殖,流式細胞術檢測DHA作用後Patu8988和SW1990細胞的凋亡、細胞週期和腫瘤相關蛋白環氧閤酶2(COX-2)的錶達量.結果 DHA作用人胰腺癌細胞株24、48、72 h後,細胞的增殖受到明顯抑製(P＜0.01),同時DHA能誘導細胞凋亡,隨著作用時間延長和作用劑量增加,效果越明顯.50μg/ml DHA作用24 h後,胰腺癌細胞的COX-2錶達量下降(P＜0.05).結論 DHA能有效地抑製胰腺癌細胞增殖,同時誘導細胞凋亡,可能與COX-2在胰腺癌細胞中的錶達下凋有關.
목적 탐토이십이탄륙희산(DHA)대인이선암세포주Patu8988화SW1990생장적작용.방법 채용MTF법검측DHA작용후Patu8988화SW1990세포적증식,류식세포술검측DHA작용후Patu8988화SW1990세포적조망、세포주기화종류상관단백배양합매2(COX-2)적표체량.결과 DHA작용인이선암세포주24、48、72 h후,세포적증식수도명현억제(P＜0.01),동시DHA능유도세포조망,수착작용시간연장화작용제량증가,효과월명현.50μg/ml DHA작용24 h후,이선암세포적COX-2표체량하강(P＜0.05).결론 DHA능유효지억제이선암세포증식,동시유도세포조망,가능여COX-2재이선암세포중적표체하조유관.
Objective To investigate the effects of docosahexaenoic acid (DHA) on the growth of human pancreatic cancer cell lines. Methods Human pancreatic cancer cell lines Patu8988 and SW1990 were treated with DHA. The cell proliferation was evaluated by MTT assay. Cell cycle, apoptosis, and cyclooxygenase-2 ex-pression were evaluated by flow cytometry. Results After incubation of pancreatic cancer cell with DHA for 24 to 72 hours, cell proliferation significantly decreased (P<0. 01) and apoptosis increased, both of which were time- dependent and dose-dependent After incubation with 50μg/ml DHA for 24 hours, cyclooxygenase-2 expression of pancreatic cancer cell lines significantly decreased (P < 0. 05). Conclusions DHA can inhibit the growth of hu- man pancreatic cancer cells via decreasing cell proliferation and inducing apoptosis. These effects may be associat- ed with the decrease of cyclooxygenase-2 expression.
