中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2012年
9期
750-753
,共4页
柯先金%李倩%朱剑%李冬梅%毛晓明%马建华%丁新生%沈洪兵
柯先金%李倩%硃劍%李鼕梅%毛曉明%馬建華%丁新生%瀋洪兵
가선금%리천%주검%리동매%모효명%마건화%정신생%침홍병
Netrin-1%骨髓间充质干细胞%血管内皮细胞生长因子%小管形成%血管生成
Netrin-1%骨髓間充質榦細胞%血管內皮細胞生長因子%小管形成%血管生成
Netrin-1%골수간충질간세포%혈관내피세포생장인자%소관형성%혈관생성
Netrin-1%Bone mesenchymal stem cells%Vascular endothelial growth factor%Tube formation%Angiogenesis
将携带增强型绿色荧光蛋白(EGFP)基因标记的Netrin-1基因重组腺病毒(Ad5-EGFP-Netrin-1)或空载体的腺病毒(Ad5-EGFP)转染到大鼠骨髓间充质干细胞后与Matrigel胶混匀共同移植到大鼠皮下,分别在移植后14d和28d观察Netrin-1和血管内皮细胞生长因子(VEGF)的蛋白表达以及微血管形成数量的情况.结果显示,Netrin-1能促进VEGF的表达和血管形成,移植的细胞数量越多而作用越强(均P＜0.05)
將攜帶增彊型綠色熒光蛋白(EGFP)基因標記的Netrin-1基因重組腺病毒(Ad5-EGFP-Netrin-1)或空載體的腺病毒(Ad5-EGFP)轉染到大鼠骨髓間充質榦細胞後與Matrigel膠混勻共同移植到大鼠皮下,分彆在移植後14d和28d觀察Netrin-1和血管內皮細胞生長因子(VEGF)的蛋白錶達以及微血管形成數量的情況.結果顯示,Netrin-1能促進VEGF的錶達和血管形成,移植的細胞數量越多而作用越彊(均P＜0.05)
장휴대증강형록색형광단백(EGFP)기인표기적Netrin-1기인중조선병독(Ad5-EGFP-Netrin-1)혹공재체적선병독(Ad5-EGFP)전염도대서골수간충질간세포후여Matrigel효혼균공동이식도대서피하,분별재이식후14d화28d관찰Netrin-1화혈관내피세포생장인자(VEGF)적단백표체이급미혈관형성수량적정황.결과현시,Netrin-1능촉진VEGF적표체화혈관형성,이식적세포수량월다이작용월강(균P＜0.05)
Rat bone mesenchymal stem cells successfully transfected by Ad5-EGFP-Netrin-1 or Ad5-EGFP were transplanted subcutaneously to SD rats after being mixed with Matrigel plug.The protein expressions of netrin-1 and vascular endothelial growth factor (VEGF),and the microvascular density were detected after 14 and 28 days.The results showed that the protein expression of VEGF and microvascular density were increased by netrin-1 treatment.The effect was enhanced when more BMSCs transfected by Ad5-EGFP-Netrin-1 were transplanted ( all P＜0.05).
