CAJ | 학술논문

目的探讨异体骨髓造血干细胞(HC)和间充质干细胞(MSC)移植对大鼠实验性结肠炎(EC)的作用.方法体外分别传代培养雄性大鼠的MSC和HC备用.实验1组的HC悬液以5-溴-2-脱氧尿嘧啶(BrdU)标记;实验2组用贴壁法获得MSC.72只雌性大鼠用三硝基苯磺酸灌肠法建立EC模型,造模后24 h,实验1组和实验2组经尾静脉分别注入HC和MSC悬浮液(每组18只),两实验组的对照组(每组18只)注入0.9%氯化钠溶液.于移植后第7、14和21天取结肠组织行病理学检查.实验1组采用SP免疫组化法检测BrdU阳性细胞,实验2组用PCR检测Y染色体的性别决定区段(sry),以确定干细胞的定位情况.结果 EC造模成功.MSC和HC培养生长迅速,均一性好.移植第7、14、21天时,实验1组病变区BrdU阳性的HC检出率均为6/6.实验2组sry阳性的MSC检出率分别为1/6、2/6和3/6.而对照组均为阴性.但两移植组的病理组织学改善均不显著.结论 HC和MSC均可在大鼠EC模型的病变肠道中定植,但移植不显著改善组织病理学,MSC定植率比HC低.
목적 탐토이체골수조혈간세포(HC)화간충질간세포(MSC)이식대대서실험성결장염(EC)적작용.방법 체외분별전대배양웅성대서적MSC화HC비용.실험1조적HC현액이5-추-2-탈양뇨밀정(BrdU)표기;실험2조용첩벽법획득MSC.72지자성대서용삼초기분광산관장법건립EC모형,조모후24 h,실험1조화실험2조경미정맥분별주입HC화MSC현부액(매조18지),량실험조적대조조(매조18지)주입0.9%록화납용액.우이식후제7、14화21천취결장조직행병이학검사.실험1조채용SP면역조화법검측BrdU양성세포,실험2조용PCR검측Y염색체적성별결정구단(sry),이학정간세포적정위정황.결과 EC조모성공.MSC화HC배양생장신속,균일성호.이식제7、14、21천시,실험1조병변구BrdU양성적HC검출솔균위6/6.실험2조sry양성적MSC검출솔분별위1/6、2/6화3/6.이대조조균위음성.단량이식조적병리조직학개선균불현저.결론 HC화MSC균가재대서EC모형적병변장도중정식,단이식불현저개선조직병이학,MSC정식솔비HC저.
Objective To investigate the effect of transplantation of allogeneic bone marrow hematopoietic cells(HCs)and mesenchymal stem cells(MSCs)on experimental colitis(EC)in rats.Methods The HCs and MSCs obtained from SD male rats were cultured and expanded in vitro.In experiment 1 and 2 groups,HCs were labeled with bromodeoxyuridine(BrdU)and MSCs were obtained using the tube wall attach technique,respectively.Seventy-two female rats were infused with trinitrobenzene sulfonic acid(TNBS)to induce EC models.After 24 hours,HC or MSC suspensions were injected into the rats in experimental 1(n=18)and 2(n=18)groups via caudal veins,respectively.Control animals were injected with isotonic saline.The whole colon was removed on day 7,14 and 21 after transplantation and examined histopathologically.BrdU labeled HCs were tested with immunohistochemical staining and MSCs were detected for sex-determining gene(sry)by PCR.Results EC models were successfully established.The HCs or MSCs grew rapidly in the culture suspension.On day 7,14 and 21 after transplantation,the BrdU immunoreactive cells were detected in the colon(6/6),and the positive expression of the sry gene was found in 1/6,2/6 and 3/6,respectively.No positive labeled cell was found in controls.There was no significant improvement in histopathological scores on the colon in two experimental groups compared with the controls.Conclusions Allogeneic HCs and MSCs may localize in the colon of EC models.The ability of localization is higher in HCs than MSCs.The transplantation of HCs and MSCs can not obviously improve histopathologically.