CAJ | 학술논문

目的探讨淋巴细胞、单核-巨噬细胞和肥大细胞在血管壁中的浸润与下肢慢性静脉功能不全( chronic venous insufficiency of low limbs,CVI)之间的关系.方法收集不同临床分期下肢慢性静脉功能不全的主干(中段)标本,C2 ～ C3级20例,C4～C6级17例,对照组(正常静脉)7例.采用免疫组织化学染色LCA,CD68和甲苯胺蓝特殊染色分别检测淋巴细胞、单核-巨噬细胞和肥大细胞表达.结果 C2 ～ C3级、C4～C6级标本中单核-巨噬细胞表达与正常对照组差异有统计学意义(F=3.56,P＜0.05)；C2 ～ C3级、C4 ～ C6级标本中肥大细胞表达与正常对照组差异有统计学意义(F =5.13,P＜0.05).结论单核-巨噬细胞和肥大细胞在血管壁的浸润可能是慢性静脉功能不全的一个重要病因.
목적 탐토림파세포、단핵-거서세포화비대세포재혈관벽중적침윤여하지만성정맥공능불전( chronic venous insufficiency of low limbs,CVI)지간적관계.방법 수집불동림상분기하지만성정맥공능불전적주간(중단)표본,C2 ～ C3급20례,C4～C6급17례,대조조(정상정맥)7례.채용면역조직화학염색LCA,CD68화갑분알람특수염색분별검측림파세포、단핵-거서세포화비대세포표체.결과 C2 ～ C3급、C4～C6급표본중단핵-거서세포표체여정상대조조차이유통계학의의(F=3.56,P＜0.05)；C2 ～ C3급、C4 ～ C6급표본중비대세포표체여정상대조조차이유통계학의의(F =5.13,P＜0.05).결론 단핵-거서세포화비대세포재혈관벽적침윤가능시만성정맥공능불전적일개중요병인.
Objective To investigate the relationship between infiltration of lymphocytes,monocyte-macrophage and mast cells in vessel wall and chronic venous insufficiency of low limbs.Methods The bole specimens (midpiece) of chronic venous insufficiency in low limbs in different clinical stages were collected,20 cases of C2- C3,17 cases of C4- C6 and 7 cases of normal venous tissue.Immunohistochemical staining and toluidine bluestaining were used to detect the expression of lymphocyte,monocyte-macrophage and mast cells.Results There were significant differences in the expression levels of monocyte-macrophage cells between C2- C3,C4- C6 specimens and normal control group ( F =3.56,P ＜ 0.05 ) ; there were significant differences in the expression levels of mast cells between C2- C3,C4- C6 specimen and normal control group ( F =5.13,P ＜ 0.05 ).Conclusions Infiltration of monocyte-macrophages and mast cells in vessel wall could be an important cause of chronic venous insufficiency.