肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2012年
8期
505-507,511
,共4页
李小毛%沈慧敏%王小韵%张旭
李小毛%瀋慧敏%王小韻%張旭
리소모%침혜민%왕소운%장욱
子宫内膜肿瘤%洛伐他汀%HEC-1-A细胞%细胞周期%G1期同步化
子宮內膜腫瘤%洛伐他汀%HEC-1-A細胞%細胞週期%G1期同步化
자궁내막종류%락벌타정%HEC-1-A세포%세포주기%G1기동보화
Endometrial neoplasms%Lovastatin%HEC-1-A cell%Cell cycle%G1 phase synchronization
目的 探讨洛伐他汀(Lov)诱导子宫内膜癌细胞株HEC-1-A G1期同步化的方法及HEC-1-A细胞解除G1期同步化后的细胞周期进程.方法 Kit-8细胞计数(CCK-8)法测定HEC-1-A细胞的倍增时间,采用10、20、30、40 μmol/L的Lov作用1×细胞倍增时间,采用流式细胞术检测G1期细胞的比例,获得G1期同步化的最佳Lov浓度;采用最佳浓度作用HEC-1-A细胞,于作用后0.5×倍增时间～2×倍增时间内,每隔4h检测同步化程度,获得最佳浓度Lov作用下的最佳同步化时间;解除G1期同步化,每隔4h检测一次,研究HEC-1-A细胞解除G1期同步化后的细胞周期进程.结果 CCK-8法检测显示HEC-1-A细胞的倍增时间约为24 h;HEC-1-A细胞的最佳G1期同步化条件为:40 μmol/L Lov作用28 h,可获取G1期细胞(87.87±0.70)％;HEC-1-A细胞于解除G1期同步化后20h,获得最大量的S期细胞,为(33.58±0.62)％;同时G1期细胞比例达到最低,为( 58.42±0.54)％.结论 40 μmol/L Lov作用28 h,可获得最大量G1期细胞;解除G1期同步化后20 h,S期细胞比例最高,G1期细胞比例达到最低,达到满意的G1期同步化效果.
目的 探討洛伐他汀(Lov)誘導子宮內膜癌細胞株HEC-1-A G1期同步化的方法及HEC-1-A細胞解除G1期同步化後的細胞週期進程.方法 Kit-8細胞計數(CCK-8)法測定HEC-1-A細胞的倍增時間,採用10、20、30、40 μmol/L的Lov作用1×細胞倍增時間,採用流式細胞術檢測G1期細胞的比例,穫得G1期同步化的最佳Lov濃度;採用最佳濃度作用HEC-1-A細胞,于作用後0.5×倍增時間～2×倍增時間內,每隔4h檢測同步化程度,穫得最佳濃度Lov作用下的最佳同步化時間;解除G1期同步化,每隔4h檢測一次,研究HEC-1-A細胞解除G1期同步化後的細胞週期進程.結果 CCK-8法檢測顯示HEC-1-A細胞的倍增時間約為24 h;HEC-1-A細胞的最佳G1期同步化條件為:40 μmol/L Lov作用28 h,可穫取G1期細胞(87.87±0.70)％;HEC-1-A細胞于解除G1期同步化後20h,穫得最大量的S期細胞,為(33.58±0.62)％;同時G1期細胞比例達到最低,為( 58.42±0.54)％.結論 40 μmol/L Lov作用28 h,可穫得最大量G1期細胞;解除G1期同步化後20 h,S期細胞比例最高,G1期細胞比例達到最低,達到滿意的G1期同步化效果.
목적 탐토락벌타정(Lov)유도자궁내막암세포주HEC-1-A G1기동보화적방법급HEC-1-A세포해제G1기동보화후적세포주기진정.방법 Kit-8세포계수(CCK-8)법측정HEC-1-A세포적배증시간,채용10、20、30、40 μmol/L적Lov작용1×세포배증시간,채용류식세포술검측G1기세포적비례,획득G1기동보화적최가Lov농도;채용최가농도작용HEC-1-A세포,우작용후0.5×배증시간～2×배증시간내,매격4h검측동보화정도,획득최가농도Lov작용하적최가동보화시간;해제G1기동보화,매격4h검측일차,연구HEC-1-A세포해제G1기동보화후적세포주기진정.결과 CCK-8법검측현시HEC-1-A세포적배증시간약위24 h;HEC-1-A세포적최가G1기동보화조건위:40 μmol/L Lov작용28 h,가획취G1기세포(87.87±0.70)％;HEC-1-A세포우해제G1기동보화후20h,획득최대량적S기세포,위(33.58±0.62)％;동시G1기세포비례체도최저,위( 58.42±0.54)％.결론 40 μmol/L Lov작용28 h,가획득최대량G1기세포;해제G1기동보화후20 h,S기세포비례최고,G1기세포비례체도최저,체도만의적G1기동보화효과.
Objective To investigate the effects of lovastatin on inducing G1 phase synchromzation in HEC-1-A cells and examine the cell cycle progression after desynchronization.Methods The doubling time of HEC-1-A cells was detected by cell counting Kit-8 assay.To determine the best lovastatin concentration of G1 synchronization,HEC-1-A cells were treated with lovastatin at concentration of 10,20,30 and 40 μmol/L respectively for 1 × doubling time,and the cell cycle was detected by flow cytometry (FCM).To determine the best period of lovastatin treatment to achieve G1 synchronization,HEC-1-A cells were treated with lovastatin at the best concentration for 0.5 × to 2 × doubling time,and the cell cycle was detected every 4 h using FCM.Furthermore,the cell cycle progress of HEC-1-A cells after desynchronization was also observed.Results The doubling time of HEC-1-A cells was 24 h.Treated with lovastatin at concentration of 40 μmol/L for 28 h achieved maximum G1 arrest (87.87±0.70) ％ in HEC-1-A cells.Minimum G1 phase (58.42±0.54) ％ and maximum S phase (33.58±0.62) ％ were observed after desynchronizing for 20 h.Conclusion Maximum G1 synchronization of HEC-1-A cells is induced by lovastatin at concentration of 40 μmol/L for 28 h.The HEC-1-A cells show minimum G1 phase and maximum S phase after desynchronizing for 20 h.
