CAJ | 학술논문

为研究苯系物暴露对工人精子染色体的损伤,用4条DNA探针与间期精子核染色体进行多色荧光原位杂交,同时检测精子1号、18号染色体数目畸变和1号染色体结构畸变(末端缺失与重复).作业车间空气中苯的时间加权浓度(TWA)为42.29 mg/m3,高于国家卫生标准(6 mg/m3).暴露组工人尿粘糠酸(ttMA)高于对照组.共计数15例暴露组工人144 282条精子,14例对照组工人135 937条精子,杂交效率为99.85%.非整倍体测定结果:暴露组精子1号、18号染色体双体率(分别为0.088%±0.041%,0.087%±0.049%)显著高于对照组1号、18号双体率(0.045%±0.024%,0.053%±0.028%);暴露组1号、18号染色体缺体率分别为(0.11%±0.059%,0.075%±0.035%)显著高于对照组相应数值(0.048%±0.018%,0.045%±0.024%);而二倍体精子率,两组差别无显著性.结构畸变测定结果:暴露组1号染色体的末端重复率、末端缺失率(分别为0.16%±0.037%,0.14%±0.053%)显著高于对照组数值(分别为0.082%±0.023%,0.069%±0.028%);暴露组1号染色体着丝粒重复率及着丝粒缺失率(0.10%±0.035%,0.10%±0.041%)显著性高于对照组相应数值(分别为0.075%±0.023%,0.060%±0.029%).实验表明,接触较高浓度苯(42.29 mg/m3)不仅可以诱导接触工人精子1,18染色体数目畸变率增高,亦可诱导生殖细胞染色体结构畸变率增高.
위연구분계물폭로대공인정자염색체적손상,용4조DNA탐침여간기정자핵염색체진행다색형광원위잡교,동시검측정자1호、18호염색체수목기변화1호염색체결구기변(말단결실여중복).작업차간공기중분적시간가권농도(TWA)위42.29 mg/m3,고우국가위생표준(6 mg/m3).폭로조공인뇨점강산(ttMA)고우대조조.공계수15례폭로조공인144 282조정자,14례대조조공인135 937조정자,잡교효솔위99.85%.비정배체측정결과:폭로조정자1호、18호염색체쌍체솔(분별위0.088%±0.041%,0.087%±0.049%)현저고우대조조1호、18호쌍체솔(0.045%±0.024%,0.053%±0.028%);폭로조1호、18호염색체결체솔분별위(0.11%±0.059%,0.075%±0.035%)현저고우대조조상응수치(0.048%±0.018%,0.045%±0.024%);이이배체정자솔,량조차별무현저성.결구기변측정결과:폭로조1호염색체적말단중복솔、말단결실솔(분별위0.16%±0.037%,0.14%±0.053%)현저고우대조조수치(분별위0.082%±0.023%,0.069%±0.028%);폭로조1호염색체착사립중복솔급착사립결실솔(0.10%±0.035%,0.10%±0.041%)현저성고우대조조상응수치(분별위0.075%±0.023%,0.060%±0.029%).실험표명,접촉교고농도분(42.29 mg/m3)불부가이유도접촉공인정자1,18염색체수목기변솔증고,역가유도생식세포염색체결구기변솔증고.