CAJ | 학술논문

小白蛋白(PV)神经元作为杏仁核簇基底外侧核(BL)中局部神经环路成分,对杏仁核的情绪、学习和记忆过程等机能发挥重要作用.为探讨BL中PV中间神经元的突触形成状态,本研究用抗PV抗体标示PV神经元,以抗多巴胺(DA)抗体标示多巴胺能轴突及末梢作为传入纤维的标志,对大鼠杏仁核做了免疫电镜双标记研究.结果表明,突触主要见于PV免疫阳性神经元的树突结构上,包括从树突干到中间及小型树突的各级分支.其中68%的突触由未标记的轴突终末形成,32%分别由DA(21%)和PV(11%)免疫阳性轴突末梢形成.PV免疫阳性神经元与未标记末梢所形成的突触大多数是对称性的,仅少数为非对称性.这些非对称性突触见于PV神经元的树突小棘和连续性突触,即一个未标记轴突末梢与另一个未标记轴突末梢形成对称性突触,后者又与PV免疫阳性神经元树突形成非对称性突触.DA和PV免疫阳性神经元轴突终末与PV免疫阳性神经元树突之间的突触全部是对称性的.以上结果表明,大鼠杏仁核BL的PV中间神经元受非对称性突触所构成的包括多巴胺系统在内的抑制性神经网络支配.
소백단백(PV)신경원작위행인핵족기저외측핵(BL)중국부신경배로성분,대행인핵적정서、학습화기억과정등궤능발휘중요작용.위탐토BL중PV중간신경원적돌촉형성상태,본연구용항PV항체표시PV신경원,이항다파알(DA)항체표시다파알능축돌급말소작위전입섬유적표지,대대서행인핵주료면역전경쌍표기연구.결과표명,돌촉주요견우PV면역양성신경원적수돌결구상,포괄종수돌간도중간급소형수돌적각급분지.기중68%적돌촉유미표기적축돌종말형성,32%분별유DA(21%)화PV(11%)면역양성축돌말소형성.PV면역양성신경원여미표기말소소형성적돌촉대다수시대칭성적,부소수위비대칭성.저사비대칭성돌촉견우PV신경원적수돌소극화련속성돌촉,즉일개미표기축돌말소여령일개미표기축돌말소형성대칭성돌촉,후자우여PV면역양성신경원수돌형성비대칭성돌촉.DA화PV면역양성신경원축돌종말여PV면역양성신경원수돌지간적돌촉전부시대칭성적.이상결과표명,대서행인핵BL적PV중간신경원수비대칭성돌촉소구성적포괄다파알계통재내적억제성신경망락지배.
As the elements of local neuronal circuits, parvalbumin (PV)-containing interneurons in the basolateral nucleus (BL) of the amygdala play an important role in the amygdaloid functions of emotion, learning and memory. In order to investigate how the PV-containing interneurons in the BL are controlled, the synapses established on PV- containing interneurons in the BL of the rat amygdala were examined under immunoelectron microscopy using the double labeling methods with anti-PV and anti-dopamine (DA) antibodies for a reference of dopaminergic axon terminals. The results show that the PV immunoreactive (IR) neurons formed the synapses mainly on the dendritic structures from shafts of the dendrites to median and small dendritic branches. 68% of the synapses on the PV-IR profiles were formed by unlabeled axon terminals, and 32 % of them were formed by DA- (21 % ) and PV- (11 % )IR axon terminals. Majority of the synapses on the PV-IR neurons formed by unlabeled axon terminals were symmetric type, and only a small a mount of them were asymmetric that were observed between the PV-IR spines and unlabeled axon terminals and in the serial synapses in which an unlabeled axon terminal symmetrically contacted to another unlabeled axon terminal that, in turn, synapsed asymmetrically to the PV-IR dendritic profiles. The synapses formed between the PV-IR profiles and DA- or PV-IR axon terminals were exclusively symmetric. The present results suggest that the PV-containing interneurons in the BL of the rat amygdala were controlled by an inhibitory network formed by the symmetric synapses around them, among which the DA system was included.