CAJ | 학술논문

DEAD盒蛋白家族的ATP依赖性的RNA解旋酶类参与细胞内几乎所有的RNA代谢过程,在几乎所有生物的细胞生长发育过程中扮演着众多不可或缺的角色.在本实验中,通过PCR和探针杂交相结合的筛选方法,筛选恶性疟原虫(Plasmodium falciparum)的基因组文库,克隆了FH1F--abstrakt同源基因的完整序列.通过搜索已经完成测序的恶性疟原虫基因组数据库,推测FH1F序列定位在第5条染色体上.FH1F全长2 804 bp,包含一个1 161 bp的完整阅读框,编码一个由386个氨基酸组成的蛋白.对FH1F蛋白序列用BlastP进行搜索和分析以及用DNAStar与许多典型的DEAD盒蛋白序列进行比对分析,结果均提示FH1F蛋白应该是DEAD盒家族的一个Abstrakt蛋白.另一方面,用DNAStar对已知所有完整的DEAD盒蛋白进行详细的序列分析以及用Mega对这些序列进行系统发育研究的结果都显示:DEAD盒家族的蛋白聚类成为若干不同的亚群;与DEAD盒蛋白的一般保守序列相比,Abstrakt,eIF-4A,Vasa,P68等不同亚群的DEAD盒蛋白在保守区具有各自不同的结构特征.本文对不同的DEAD盒蛋白的结构特征进行了总结并试图给出不同亚群分类上的结构标准,对Abstrakt蛋白在本应高度保守的位点上异常于其它DEAD盒蛋白的氨基酸残基的取代也进行了相关的初步分析[动物学报 50(3):420-430,2004].
DEAD합단백가족적ATP의뢰성적RNA해선매류삼여세포내궤호소유적RNA대사과정,재궤호소유생물적세포생장발육과정중분연착음다불가혹결적각색.재본실험중,통과PCR화탐침잡교상결합적사선방법,사선악성학원충(Plasmodium falciparum)적기인조문고,극륭료FH1F--abstrakt동원기인적완정서렬.통과수색이경완성측서적악성학원충기인조수거고,추측FH1F서렬정위재제5조염색체상.FH1F전장2 804 bp,포함일개1 161 bp적완정열독광,편마일개유386개안기산조성적단백.대FH1F단백서렬용BlastP진행수색화분석이급용DNAStar여허다전형적DEAD합단백서렬진행비대분석,결과균제시FH1F단백응해시DEAD합가족적일개Abstrakt단백.령일방면,용DNAStar대이지소유완정적DEAD합단백진행상세적서렬분석이급용Mega대저사서렬진행계통발육연구적결과도현시:DEAD합가족적단백취류성위약간불동적아군;여DEAD합단백적일반보수서렬상비,Abstrakt,eIF-4A,Vasa,P68등불동아군적DEAD합단백재보수구구유각자불동적결구특정.본문대불동적DEAD합단백적결구특정진행료총결병시도급출불동아군분류상적결구표준,대Abstrakt단백재본응고도보수적위점상이상우기타DEAD합단백적안기산잔기적취대야진행료상관적초보분석[동물학보 50(3):420-430,2004].
The ATP-dependent RNA helicases of the DEAD-box family, involved in almost every cellular process of RNA metabolism, play many essential roles during cell development and growth in almost all organisms. In this study,FH1F***, the full-length gene of a putative Abstrakt, was isolated by screening the genomic library of Plasmodium falciparum . FH1F was inferred located in the chromosome 5 in the whole genome. FH1F is composed of 2 804 bp and encodes a predicted protein of 386 aa. Alignment analysis of the predicted protein sequence with many typical DEAD-box proteins suggested FH1F protein was an Abstrakt of DEAD-box family. By phylogenetic analysis we realized that DEADbox proteins assembled into different subgroups. Then we listed different organization of conserved motifs in Abstrakt,eIF-4A, Vasa, P68 proteins as compared to the general DEAD-box protein consensus, and pointed out the novel residue substitutions in Abstrakt proteins in the highly conserved positions. Our analysis will be important for providing general insights into the structural characteristics of different DEAD-box proteins [ Acta Zoologica Sinica 50 (3): 420 - 430,2004].