CAJ | 학술논문

GATA基因在脊椎动物和非脊椎动物的发育中行使重要的功能,该家族的成员在进化上也足非常保守的.脊椎动物的GATA基因分为两个亚群:GATA1/2/3和GATA4/5/6.通过生物信息分析,在文吕鱼的基因缓中找到了3个GATA基因:一个GATA1/2/3业家族基因,两个GATA4/5/6亚家族基因:还找到一个类GATA基因.还克隆了白氏文昌鱼(Branchiostoma belcheri)GATA123的一段序列,并研究了它在早期胚胎发育中的表达图式.结果表明GATA123在原肠胚的中内胚层表达,而在神经胚晚期和幼体早期,GATA123在脑泡和消化道中部区域表达.这种表达模式与头部发育的重要基因Otx相类似.结果提示在文吕鱼脑泡的发育过程中GATA123和Otx很可能共同发挥着重要的作用.
GATA기인재척추동물화비척추동물적발육중행사중요적공능,해가족적성원재진화상야족비상보수적.척추동물적GATA기인분위량개아군:GATA1/2/3화GATA4/5/6.통과생물신식분석,재문려어적기인완중조도료3개GATA기인:일개GATA1/2/3업가족기인,량개GATA4/5/6아가족기인:환조도일개류GATA기인.환극륭료백씨문창어(Branchiostoma belcheri)GATA123적일단서렬,병연구료타재조기배태발육중적표체도식.결과표명GATA123재원장배적중내배층표체,이재신경배만기화유체조기,GATA123재뇌포화소화도중부구역표체.저충표체모식여두부발육적중요기인Otx상유사.결과제시재문려어뇌포적발육과정중GATA123화Otx흔가능공동발휘착중요적작용.
GATA factors are evolutionarily conserved and play crucial roles during embryonic development in both vertebrates and invertebrates. Vertebrate GATAs can be divided into two subgroups, the GATA1/2/3 and the GATA4/5/6 classes. Through genomic analysis, we have identified three GATA factors, representing the GATA1/2/3 and GATA4/5/6 subfamilies respectively, and one GATA like protein in the genome of the basal chordate amphioxus (Branchiostoma floridae, cephalochordata). Partial sequence of GATA123 in the amphioxus Branchiostoma belcheri (BbGATA123) was cloned and its expression pattern during early embryonic development was studied. Expression of BbGATA123 is first detected in the mesendoderm during gastrulation. Interestingly, in the late neurula and early larva stages, it is expressed strongly in the cerebral vesicle and the mid gut region. Its expression is compared to Otx, a gene known crucial for the development of anterior structures. Our observations suggest that GATA123, together with Otx, might play an important role in the development of amphioxus cerebral vesicle, the counterpart of the vertebrate brain.