国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2009年
5期
459-460
,共2页
尿紧张素类%先兆子痫%血清%胎盘
尿緊張素類%先兆子癇%血清%胎盤
뇨긴장소류%선조자간%혈청%태반
Urotensins%Pre-eclampsia%Serum%placenta
目的 通过子痫前期孕妇和新生儿脐血尿紧张素Ⅱ(UⅡ)检测和UⅡ mRNA在胎盘组织的表达水平,探索其在子痫前期发生、发展中的作用.方法 1)应用ELISA方法对40例子痫前期患者及20例同期正常妊娠孕妇(健康对照组)外周血和新生儿脐血进行血清UⅡ测定.2)同时用RT-PCR检测胎盘组织中UⅡmRNA的表达水平.结果 1)子痫前期组血清UⅡ浓度明显高于健康对照组,差异有统计学意义(P<0.05).2)胎盘组织UⅡmRNA表达子痫前期组明显高于健康对照组,差异有统计学意义(P<0.05).结论 UⅡ在胎盘组织缺血缺氧和动脉粥样硬化的发生中起重要作用.
目的 通過子癇前期孕婦和新生兒臍血尿緊張素Ⅱ(UⅡ)檢測和UⅡ mRNA在胎盤組織的錶達水平,探索其在子癇前期髮生、髮展中的作用.方法 1)應用ELISA方法對40例子癇前期患者及20例同期正常妊娠孕婦(健康對照組)外週血和新生兒臍血進行血清UⅡ測定.2)同時用RT-PCR檢測胎盤組織中UⅡmRNA的錶達水平.結果 1)子癇前期組血清UⅡ濃度明顯高于健康對照組,差異有統計學意義(P<0.05).2)胎盤組織UⅡmRNA錶達子癇前期組明顯高于健康對照組,差異有統計學意義(P<0.05).結論 UⅡ在胎盤組織缺血缺氧和動脈粥樣硬化的髮生中起重要作用.
목적 통과자간전기잉부화신생인제혈뇨긴장소Ⅱ(UⅡ)검측화UⅡ mRNA재태반조직적표체수평,탐색기재자간전기발생、발전중적작용.방법 1)응용ELISA방법대40례자간전기환자급20례동기정상임신잉부(건강대조조)외주혈화신생인제혈진행혈청UⅡ측정.2)동시용RT-PCR검측태반조직중UⅡmRNA적표체수평.결과 1)자간전기조혈청UⅡ농도명현고우건강대조조,차이유통계학의의(P<0.05).2)태반조직UⅡmRNA표체자간전기조명현고우건강대조조,차이유통계학의의(P<0.05).결론 UⅡ재태반조직결혈결양화동맥죽양경화적발생중기중요작용.
Objective To explore the effect of urotensin Ⅱ (U Ⅱ) in occurrence and development of pre-eclampsia through the examination of U Ⅱ in pre-eclampsia pregnant woman and newborn cord blood and the expression of U Ⅱ mRNA in placenta. Methods 1) The serum U Ⅱ of peripheral blood and newborn cord blood was determined in 40 pre-eclampsia patients and 20 normal pregnant women (control group) by using ELISA method. 2) The U Ⅱ mRNA expression level in placenta tissue was simultaneously examined with RT-PCR method. Results The concentration o{ serum U Ⅱ in pre-eclampia patients was obviously higher than that in control group (P<0.05).2) The U Ⅱ mRNA ex-pression level in placenta tissue in pre-eclampsia patients was obviously higher than that in control group (P<0.05). Conclusion U Ⅱ plays an important role in development of ischemia, anoxemia and atherosclerosis of placenta tissue.
