家畜生态学报
傢畜生態學報
가축생태학보
ACTA ECOLOGAE ANIMALIS DOMASTICI
2009年
4期
14-17
,共4页
单雪松%陈洋%崔庆媛%李克鹏
單雪鬆%陳洋%崔慶媛%李剋鵬
단설송%진양%최경원%리극붕
MHD2基因%杂种优势%mRNA差异显示%鸭%番鸭
MHD2基因%雜種優勢%mRNA差異顯示%鴨%番鴨
MHD2기인%잡충우세%mRNA차이현시%압%번압
MHD2 gene%hybrid vigor%mRNA differential display%duck%muscovy duck
以北京鸭、法国番鸭及其正反交F1代杂种为试验材料,采用mRNA差异显示(DDRT-PCR)方法分析不同试验组肌肉组织基因表达差异,结合抑制性消减杂交(SSH)加以验证,发现一个杂种下调表达基因r-MHD2,对鸭r-MHD2基因698 bp片段BLASTING结果表明,该基因与鸡(Gallus gallus)、非洲爪蟾Xenopus laevis和Xenopus tropicalis同源性分别为91%、78%和77%.r-MHD2基因的具体分子功能尚属未知.
以北京鴨、法國番鴨及其正反交F1代雜種為試驗材料,採用mRNA差異顯示(DDRT-PCR)方法分析不同試驗組肌肉組織基因錶達差異,結閤抑製性消減雜交(SSH)加以驗證,髮現一箇雜種下調錶達基因r-MHD2,對鴨r-MHD2基因698 bp片段BLASTING結果錶明,該基因與鷄(Gallus gallus)、非洲爪蟾Xenopus laevis和Xenopus tropicalis同源性分彆為91%、78%和77%.r-MHD2基因的具體分子功能尚屬未知.
이북경압、법국번압급기정반교F1대잡충위시험재료,채용mRNA차이현시(DDRT-PCR)방법분석불동시험조기육조직기인표체차이,결합억제성소감잡교(SSH)가이험증,발현일개잡충하조표체기인r-MHD2,대압r-MHD2기인698 bp편단BLASTING결과표명,해기인여계(Gallus gallus)、비주조섬Xenopus laevis화Xenopus tropicalis동원성분별위91%、78%화77%.r-MHD2기인적구체분자공능상속미지.
The gene expression of muscle tissue from Beijing duck, France muscovy duck and their mutual hybrids were examined with the method of DDRT-PCT, and the display result was verified by the method of SSH.One hybrid down-regulated gene r-MHD2 was found in this research.The sequencing and blasting result of the 698bp fragment of duck r-MHD2 gene showed that the identity of this gene and Gallus gallus, Xenopus laevis and Xenopus tropicalisgene is 91%, 78% and 77% respectively.
