世界华人消化杂志
世界華人消化雜誌
세계화인소화잡지
WORLD CHINESE JOURNAL OF DIGESTOLOGY
2009年
18期
1883-1887
,共5页
NDRG1%肝细胞癌%胎肝%免疫组织化学
NDRG1%肝細胞癌%胎肝%免疫組織化學
NDRG1%간세포암%태간%면역조직화학
N-myc downstream regulated gene 1%Hepatocellular carcinoma%Fetal liver%Immunohistochemistry
目的:探讨NDRG1在原发性肝细胞癌(HCC)及胎肝组织中的表达及其意义.方法:收集2002-01/2008-12广州市第一人民医院手术切除的肝细胞癌标本81例. 所有患者术前未行放疗和化疗; 25例胎肝组织, 取自不同月份流产或引产的胎儿(4、5、6、7、8 mo胎儿各5例); 另选43例癌旁组织, 10例肝硬化组织, 9例正常肝组织(移植肝), 8例原发癌转移灶组织作为对照. 观察肝脏组织病理形态特征, 并用免疫组织化学EnVison法检测NDRG1的表达.结果:NDRG1在正常肝组织中呈强阳性表达, 平均吸光度值为0.206±0.056, 随着肿瘤的发生, 在癌旁组织中有减弱(0.176±0.083),在HCC中表达明显减弱(0.128±0.096), 在转移灶中表达最低(0.059±0.051), 而在胎肝组织中表达亦较低(0.059±0.074). 各组总体差异均有统计学意义(F = 33.669, P <0.05). HCC与患者年龄、性别、肝炎病史、肝硬化、肿瘤大小、AFP值、HbsAg、淋巴结转移及有无远处转移、肿瘤分型、Child-Pugh分级、TNM分期、CLIP分期均无关(P >0.05), 但与肿瘤的Edmondson分级有关(F = 2.881, P <0.05).结论:NDRG1在HCC中低表达, 并且随着肿瘤的发生发展, 表达量逐渐降低. NDRG1可能对HCC起着抑制作用, 提示该基因可望成为早期预测肝癌转移的分子生物学标志物之一.
目的:探討NDRG1在原髮性肝細胞癌(HCC)及胎肝組織中的錶達及其意義.方法:收集2002-01/2008-12廣州市第一人民醫院手術切除的肝細胞癌標本81例. 所有患者術前未行放療和化療; 25例胎肝組織, 取自不同月份流產或引產的胎兒(4、5、6、7、8 mo胎兒各5例); 另選43例癌徬組織, 10例肝硬化組織, 9例正常肝組織(移植肝), 8例原髮癌轉移竈組織作為對照. 觀察肝髒組織病理形態特徵, 併用免疫組織化學EnVison法檢測NDRG1的錶達.結果:NDRG1在正常肝組織中呈彊暘性錶達, 平均吸光度值為0.206±0.056, 隨著腫瘤的髮生, 在癌徬組織中有減弱(0.176±0.083),在HCC中錶達明顯減弱(0.128±0.096), 在轉移竈中錶達最低(0.059±0.051), 而在胎肝組織中錶達亦較低(0.059±0.074). 各組總體差異均有統計學意義(F = 33.669, P <0.05). HCC與患者年齡、性彆、肝炎病史、肝硬化、腫瘤大小、AFP值、HbsAg、淋巴結轉移及有無遠處轉移、腫瘤分型、Child-Pugh分級、TNM分期、CLIP分期均無關(P >0.05), 但與腫瘤的Edmondson分級有關(F = 2.881, P <0.05).結論:NDRG1在HCC中低錶達, 併且隨著腫瘤的髮生髮展, 錶達量逐漸降低. NDRG1可能對HCC起著抑製作用, 提示該基因可望成為早期預測肝癌轉移的分子生物學標誌物之一.
목적:탐토NDRG1재원발성간세포암(HCC)급태간조직중적표체급기의의.방법:수집2002-01/2008-12엄주시제일인민의원수술절제적간세포암표본81례. 소유환자술전미행방료화화료; 25례태간조직, 취자불동월빈유산혹인산적태인(4、5、6、7、8 mo태인각5례); 령선43례암방조직, 10례간경화조직, 9례정상간조직(이식간), 8례원발암전이조조직작위대조. 관찰간장조직병리형태특정, 병용면역조직화학EnVison법검측NDRG1적표체.결과:NDRG1재정상간조직중정강양성표체, 평균흡광도치위0.206±0.056, 수착종류적발생, 재암방조직중유감약(0.176±0.083),재HCC중표체명현감약(0.128±0.096), 재전이조중표체최저(0.059±0.051), 이재태간조직중표체역교저(0.059±0.074). 각조총체차이균유통계학의의(F = 33.669, P <0.05). HCC여환자년령、성별、간염병사、간경화、종류대소、AFP치、HbsAg、림파결전이급유무원처전이、종류분형、Child-Pugh분급、TNM분기、CLIP분기균무관(P >0.05), 단여종류적Edmondson분급유관(F = 2.881, P <0.05).결론:NDRG1재HCC중저표체, 병차수착종류적발생발전, 표체량축점강저. NDRG1가능대HCC기착억제작용, 제시해기인가망성위조기예측간암전이적분자생물학표지물지일.
AIM: To investigate the expression of NDRG1 in primary hepatocellular carcinoma (HCC) and fetal liver and its significance. METHODS: Eighty one surgical resection specimens of hepatocellular carcinoma were obtained during 2002-01 and 2008-12, and all patients did not undergo pre-operative radiotherapy or chemotherapy. Twenty five fetal liver tissue specimens were taken from fetuses at different months (4, 5, 6, 7, 8 mo of the five cases of fetus). Forty three cases of para-carcinoma tissues, 10 cases of liver cirrhosis tissue, 9 cases of normal liver tissue (liver transplantation), and 8 cases of primary cancer metastasis tissue were taken as the controls. The pathological morphological characteristics of liver tissue were observed, and the expression of NDRG1 was detected using immunohistochemical EnVision. RESULTS: NDRG1 expression in normal liver tissue was strongly positive, and the average optical density value was 0.206 ± 0.056. With the progress of carcinoma, NDRG1 expression was weakened in para-carcinoma tissues, and the average optical density value was 0.176 ± 0.083. It was decreased significantly in the HCC (0.128 ± 0.096), bottomed in metastasis focus (0.059 ± 0.051), and was also lower in fetal liver tissue (0.059 ± 0.074). The overall differences among the groups were statistically significant (F = 33.669, P < 0.05). HCC was not related to the age, sex, history of hepatitis, with or without cirrhosis, carcinoma size, AFP value, HbsAg, with or without lymph node metastasis, or distant metastasis, carcinoma type, Child-Pugh classification, TNM staging , CLIP stages (P > 0.05), but related to the Edmondson classification of carcinoma (F = 2.881, P < 0.05). CONCLUSION: Low-expression of NDRG1 in HCC is observed and the expression is further decreased with the tumor development and progression. Therefore, NDRG1 exerts an inhibitory effect on HCC, suggesting that the gene is expected to become a molecular marker for the early prediction of HCC metastasis.