CAJ | 학술논문

在浸润条件下,以0.5%(v/v)戊二醛交联的高分子膜尼龙载体固定化木瓜蛋白酶.对固定化条件进行了优化,比较了固定化酶与游离酶的酶学参数.结果表明,4℃、pH 6.0条件下,将膜载体浸润于2 mg/mL酶液中5 h,固定化酶活为303.4 U/g.固定化酶最适反应pH为6.0～7.0,最适反应温度为65℃.其pH稳定性、热稳定性均比游离酶高.
재침윤조건하,이0.5%(v/v)무이철교련적고분자막니룡재체고정화목과단백매.대고정화조건진행료우화,비교료고정화매여유리매적매학삼수.결과표명,4℃、pH 6.0조건하,장막재체침윤우2 mg/mL매액중5 h,고정화매활위303.4 U/g.고정화매최괄반응pH위6.0～7.0,최괄반응온도위65℃.기pH은정성、열은정성균비유리매고.
Papain was immobilized on 0.5% (v/v) glutaraldehyde cross-linked nylon membrane by soaking method.The optimum immobilized conditions and the characteristics of free and immobilized papain were determined.The results showed that in pH 6.0,the carder was soaked in 2 mg/mL papain solution at 4℃ for 5 h,the activity of immobilized papain 303.4U/g.The pH and temperature of optimum reaction of immobilized papain were 6.0-7.0 and 65℃.The pH and thermal stabilities of immobilized papain were higher than those of free papain.