CAJ | 학술논문

背景:目前研究多为骨髓间充质干细胞的体外培养及细胞移植对颅内疾病的治疗,对植入细胞在损伤脊髓中的成活、分化、迁移、结构重建等了解有限.目的:探讨局部骨髓间充质干细胞移植在脊髓损伤修复中的作用和骨髓间充质干细胞替代治疗的可行性.方法:成年健康雌性SD大鼠随机分为细胞移植组和对照组,建立SD大鼠脊髓横断损伤模型,伤后即刻分别向损伤区局部移植大鼠骨髓间充质干细胞悬液或无钙镁磷酸缓冲液.在术前和术后1 d,1周,2周,3周,4周和8周进行BBB评分,观测大鼠的运动功能,并于移植后1周免疫组织化学染色法观察BrdU标记的骨髓间充质干细胞在脊髓损伤处的存活情况,移植后4周进行损伤脊髓的大体观察和组织学检测.结果与结论:移植后第1～8周细胞移植组BBB评分均高于对照组;术后1周免疫组织化学染色结果显示在细胞移植组大鼠脊髓远端检测到BrdU阳性细胞,术后4周脊髓损伤处发现有神经纤维.证实通过损伤后立即局部注射的方式将骨髓间充质干细胞移植进大鼠脊髓损伤区,细胞可在损伤区存活;存活的骨髓间充质干细胞可分化为神经元,在损伤局部形成神经元通路,从而促进脊髓神经纤维传导功能的恢复,并促进高位脊髓损伤后大鼠后肢运动功能恢复.
배경:목전연구다위골수간충질간세포적체외배양급세포이식대로내질병적치료,대식입세포재손상척수중적성활、분화、천이、결구중건등료해유한.목적:탐토국부골수간충질간세포이식재척수손상수복중적작용화골수간충질간세포체대치료적가행성.방법:성년건강자성SD대서수궤분위세포이식조화대조조,건립SD대서척수횡단손상모형,상후즉각분별향손상구국부이식대서골수간충질간세포현액혹무개미린산완충액.재술전화술후1 d,1주,2주,3주,4주화8주진행BBB평분,관측대서적운동공능,병우이식후1주면역조직화학염색법관찰BrdU표기적골수간충질간세포재척수손상처적존활정황,이식후4주진행손상척수적대체관찰화조직학검측.결과여결론:이식후제1～8주세포이식조BBB평분균고우대조조;술후1주면역조직화학염색결과현시재세포이식조대서척수원단검측도BrdU양성세포,술후4주척수손상처발현유신경섬유.증실통과손상후립즉국부주사적방식장골수간충질간세포이식진대서척수손상구,세포가재손상구존활;존활적골수간충질간세포가분화위신경원,재손상국부형성신경원통로,종이촉진척수신경섬유전도공능적회복,병촉진고위척수손상후대서후지운동공능회복.
BACKGROUND:Present studies mainly focused on in vitro culture of bone marrow mesenchymal stem cells(BMSCs)and cell transplantation for treating intracalvarium diseases.However,the understanding of survival,differentiation,migration and structure of transplanted cells in the damaged spinal cord is limited.OBJECTIVE:To explore effects of local BMSC transplantation in repair of spinal cord damage and feasibility of replacement therapy of BMSCs.METHODS:Adult healthy female Sprague-Dawley rats were randomly assigned to cell transplantation and control groups.Rat models of spinal cord transection damage were established.Rat BMSC suspension or calcium and magnesium phosphate buffer were transplanted immediately after injury to the damage zone.At 1 day,1,2,3,4 and 8 weeks before and after transplantation,BBB score motor function was observed in rats,and at 1 week after transplantation,immunohistochemical staining was utilized to observe BrdU-labeled BMSC survival in the spinal cord damaged site.At 4 weeks after transplantation,the general observation and histological detection were observed.RESULTS AND CONCLUSION:At 1-8 weeks after transplantation,BBB scores were higher in the cell transplantation group than in the control group.At 1 week following surgery,immunohistochemical staining showed that BrdU-positive cells were detected in the distal end of rat spinal cord in the cell transplantation group.At 4 weeks following surgery,nerve fibers were found in the damaged spinal cord.These verified that BMSCs were transplanted into rat damaged spinal cord immediately following damage,and the transplanted cells could survive.Living BMSCs can differentiate into neurons,and formed neuron pathway in the local region of damage,which will promote the recovery of conduction function of spinal nerve fibers,and contribute to the recovery of rat hindlimb motor function following high-level spinal cord injury.