CAJ | 학술논문

目的研究汉防己碱(Tet)用于治疗重症急性胰腺炎(SAP)作用及其机制.方法将45只SD大鼠随机分为对照组、重症急性胰腺炎组(SAP组)及汉防已碱组(Tet组).各组术后3 h、6 h、12 h随机取5只大鼠,开腹取材检测.检测血钙离子浓度,胰腺腺泡内钙离子荧光强度及胰腺病理评分.结果对照组检测指标在组内各时间段数值比较无统计学意义(P＞0.05).SAP组和Tet组检测在组内各时间段及组间比较均有统计学意义(P＜0.05).结论本实验证实Tet作为钙离子的阻滞剂可明显抑制钙离子进入胰腺腺泡细胞,有效减轻实验大鼠胰腺的病理损害;钙超载在SAP发展中起重要作用.
목적 연구한방기감(Tet)용우치료중증급성이선염(SAP)작용급기궤제.방법 장45지SD대서수궤분위대조조、중증급성이선염조(SAP조)급한방이감조(Tet조).각조술후3 h、6 h、12 h수궤취5지대서,개복취재검측.검측혈개리자농도,이선선포내개리자형광강도급이선병리평분.결과 대조조검측지표재조내각시간단수치비교무통계학의의(P＞0.05).SAP조화Tet조검측재조내각시간단급조간비교균유통계학의의(P＜0.05).결론 본실험증실Tet작위개리자적조체제가명현억제개리자진입이선선포세포,유효감경실험대서이선적병리손해;개초재재SAP발전중기중요작용.
Objective To study the effect and mechanism of tetrandrine (Tet) treatment of severe acute pancreatitis (SAP). Methods 45 SD rats were randomly divided into 3 groups: control group, severe acute pancreatitis group (SAP group) and tetrandrine group (Tet group). 5 rats were randomly taken from each group 3, 6, and 12 h after operation, and then underwent laparotomy for testing. Detection targets included calciumion concentration, pancreatic acinar calcium fluorescence intensity and pancreas pathological evaluation. Results In terms of the value of all detection targets, there was no statistical difference for the control group between different time points (P ＞0.05 )while there was statistical difference for SAP group and TET group in different time points (P＜0.05). Conclusion The experiment confirms that Tet, as a calcium blocker, can inhibit calcium to penetrate into pancreatic acinar cells, thus effectively reduce the pathological damage to pancreas of the experimental rats. Calcium overload plays an important role in SAP development.