CAJ | 학술논문

目的探讨EphA2、EphrinAl及E-cadherin在胰腺癌及癌旁非肿瘤组织中的表达及临床意义.方法.采用免疫组织化学EnVision二步法,检测48例胰腺癌及癌旁非肿瘤胰腺组织中EphA 2、EphrinAl和E-cadherin的表达,并分析其与临床病理因素的关系.应用log-rank检验和Cox比例风险模型分析EphA 2、EphrinAl和E-cadherin的表达与患者预后的关系.结果 EphA 2、EphrinAl和E-cadherin在胰腺癌组织和癌旁非肿瘤组织阳性表达差异均有统计学意义.Ⅲ～Ⅳ期胰腺癌EphA 2、EphrinAl强阳性染色及E-cadherin阴性染色分别为47.9%、47.9%和64.6%,显著高于Ⅰ～Ⅱ期的6.25%、8.3%和14.6%(P<0.05).E-cadherin表达阳性与阴性患者术后生存率比较差异有统计学意义(P<0.05).Cox多因素分析表明,JPS分期、EphA 2阳性表达及E-cadherin阴性表达是反映胰腺癌预后的独立指标.结论 EphA2/EphrinAl与E-cadherin蛋白表达异常可能共同参与了胰腺癌的发生、发展与转移;联合检测三种蛋白对于评价胰腺癌的预后有一定参考价值.
목적 탐토EphA2、EphrinAl급E-cadherin재이선암급암방비종류조직중적표체급림상의의.방법.채용면역조직화학EnVision이보법,검측48례이선암급암방비종류이선조직중EphA 2、EphrinAl화E-cadherin적표체,병분석기여림상병리인소적관계.응용log-rank검험화Cox비례풍험모형분석EphA 2、EphrinAl화E-cadherin적표체여환자예후적관계.결과 EphA 2、EphrinAl화E-cadherin재이선암조직화암방비종류조직양성표체차이균유통계학의의.Ⅲ～Ⅳ기이선암EphA 2、EphrinAl강양성염색급E-cadherin음성염색분별위47.9%、47.9%화64.6%,현저고우Ⅰ～Ⅱ기적6.25%、8.3%화14.6%(P<0.05).E-cadherin표체양성여음성환자술후생존솔비교차이유통계학의의(P<0.05).Cox다인소분석표명,JPS분기、EphA 2양성표체급E-cadherin음성표체시반영이선암예후적독립지표.결론 EphA2/EphrinAl여E-cadherin단백표체이상가능공동삼여료이선암적발생、발전여전이;연합검측삼충단백대우평개이선암적예후유일정삼고개치.
Objective To investigate the relationship between EphA2, EphrinAl and E-cadherin expressions and tumor stage and prognosis in pancreatic cancer. Method EphA2, EphrinAl and Ecadherin expressions were detected by immunohistochemistry in the tumor tissue and normal tissue specimens from 48 patients with primary pancreatic cancer. Results The expressions of EphA2 and EphrinAl were higher in the pancreatic carcinoma tissues than in the normal pancreatic tissues (P<0. 05). The E-cadherin expression was lower in the pancreatic cancer tissues than in the normal pancreatic tissues (P<0. 05). With decreasing histological differentiation, the expressions of EphA2 and EphrinAl in carcinoma tissues increased significantly (P<0. 05), while the E-cadherin expression decreased significantly (P<0. 05). The positive expressions of EphA2 and EphrinAl in the primary tumor significantly increased in stageⅢ and Ⅳ than in stage Ⅰ and Ⅱ (47. 9%vs 6. 25% , P<0. 05;47. 9% vs 8. 3%, P<0. 05), while the negative expression of E-cadherin was reversely correlated with these tumor stages (14. 6% vs 64. 6%, P<0. 05). Cox multivariate analysis showed that the clinical stage, EphA2 positive expression and E-cadherin negative expression were significantly associated with survival. Conclusion Abnormal expressions of EphA2, EphrinAl and E-cadherin were involved in the progression of pancreatic cancer and they were useful in predicting prognosis.