CAJ | 학술논문

目的探讨矿化明胶海绵作为支架材料在骨组织工程应用中的可行性.方法应用醋酸钙和磷酸钠试剂分别对可吸收明胶海绵用沉积(P)和微波炉法进行表面矿化,运用扫描电镜、X线衍射、傅立叶红外衍射对表面改良的明胶海绵进行理化性能检测.动物实验:新西兰兔颅骨缺损动物模型,制备6 mm直径大小的全颅层缺损区,4、8、12周组织形态学观察分析比较矿化和未矿化的明胶海绵及临床上常用骨移植材料Bio-oss的成骨效果.结果扫描电镜显示表面处理明胶海绵表面均匀被覆粗糙膜状物质,X线衍射、傅立叶红外衍射显示表面处理明胶海绵具有磷酸灰石成分.微波炉法优于沉积法.组织切片显示矿化明胶海绵支架有良好的组织相容性.在材料周围有大量的未矿化的新生骨,同时有很多成骨细胞和破骨细胞存在,能有效修复新西兰兔的颅骨缺损区.结论微波炉法是一种简单高效的表面涂层技术,表面改良的明胶海绵作为组织工程支架材料具有广阔的发展前景.
목적 탐토광화명효해면작위지가재료재골조직공정응용중적가행성.방법 응용작산개화린산납시제분별대가흡수명효해면용침적(P)화미파로법진행표면광화,운용소묘전경、X선연사、부립협홍외연사대표면개량적명효해면진행이화성능검측.동물실험:신서란토로골결손동물모형,제비6 mm직경대소적전로층결손구,4、8、12주조직형태학관찰분석비교광화화미광화적명효해면급림상상상용골이식재료Bio-oss적성골효과.결과 소묘전경현시표면처리명효해면표면균균피복조조막상물질,X선연사、부립협홍외연사현시표면처리명효해면구유린산회석성분.미파로법우우침적법.조직절편현시광화명효해면지가유량호적조직상용성.재재료주위유대량적미광화적신생골,동시유흔다성골세포화파골세포존재,능유효수복신서란토적로골결손구.결론 미파로법시일충간단고효적표면도층기술,표면개량적명효해면작위조직공정지가재료구유엄활적발전전경.
Objective To explore the feasibility of the preparation of a new scaffold material by mineralizing with calcium phosphate.Methods The collagen sponges were mineralized with calcium acetate and sodium phosphate reagents by using precipitation method and microwave method respectively.The surface characteristics of surface-modified collagen sponges were analyzed by scanning electron microscopy ( SEM),X-ray diffractometer (XRD) and fourier transform infrared spectrometer (FT-IR).A pilot was made with cranial defect model.Then 6 mm diameter defects were created.The histomorphometric surface-modified collagen sponges were compared with collagen sponges and Bio-Oss after 4,8,12 weeks.Results SEM scanning indicated that collagen sponges were mostly coated with calcium phosphate by the precipitation method and the microwave method.Carbonate apatite existed.Microwave method was better than precipitation method.The histological observations showed calcified newly formed bone surrounding surface-modified collagen sponges.Massive osteocytes existed.Conclusions The collagen sponges mineralized by calcium phosphate has better properties of bone formation.Surface-modified collagen sponge as a scaffold material is a promising biomaterial.