CAJ | 학술논문

目的观察结扎新西兰大白兔双侧颈总动脉导致基底动脉分叉处动脉瘤的形成,探讨该过程中动脉分叉处内皮细胞的形态和功能变化.方法将15只新西兰大白兔分成实验组和对照组.实验组大白兔结扎双侧颈总动脉代偿性地提高基底动脉的血流,对照组行假手术.在结扎颈总动脉后的3个月内,通过多普勒监测基底动脉的血流.1.5、3个月后,获取兔基底动脉分叉部,采用电子显微镜和免疫组织化学的方法观察分叉部内皮细胞的形态和功能.结果在第1次手术后的0、1、4、7、14、28、35、42、49、56、70、84 d后,采用多普勒测量观察到实验组基底动脉血流量较对照组显著增加.在实验组中,首次结扎的1.5个月后,β-catenin仅表达在内皮细胞核,3个月后,在兔的基底动脉分叉处出现动脉瘤的早期阶段,并未见有β-catenin的表达.电镜下观察到结扎的1.5个月后的内皮细胞由梭形变成柱形,3个月后内皮细胞间出现裂缝,并出现细胞核边集.结论内皮细胞的损伤诱发了脑动脉瘤的形成.
목적 관찰결찰신서란대백토쌍측경총동맥도치기저동맥분차처동맥류적형성,탐토해과정중동맥분차처내피세포적형태화공능변화.방법 장15지신서란대백토분성실험조화대조조.실험조대백토결찰쌍측경총동맥대상성지제고기저동맥적혈류,대조조행가수술.재결찰경총동맥후적3개월내,통과다보륵감측기저동맥적혈류.1.5、3개월후,획취토기저동맥분차부,채용전자현미경화면역조직화학적방법관찰분차부내피세포적형태화공능.결과 재제1차수술후적0、1、4、7、14、28、35、42、49、56、70、84 d후,채용다보륵측량관찰도실험조기저동맥혈류량교대조조현저증가.재실험조중,수차결찰적1.5개월후,β-catenin부표체재내피세포핵,3개월후,재토적기저동맥분차처출현동맥류적조기계단,병미견유β-catenin적표체.전경하관찰도결찰적1.5개월후적내피세포유사형변성주형,3개월후내피세포간출현렬봉,병출현세포핵변집.결론 내피세포적손상유발료뇌동맥류적형성.
Objective To investigates the morphological and function changes of the endothelial cells at the rabbit basilar bifurcation in response to sustained high blood flow after bilateral common carotid artery ligation. Methods Fifteen adult female New Zealand white rabbits were randomly divided into ex-perimental group and sham control group. The rabbits in experiment group were subjected to bilateral com-mon carotid artery ligation to increase the compensatory basilar artery flow, and those in control group got sham operation. Basilar artery flow was monitored by transcranial Doppler within 3 months after surgery. Basilar artery bifurcation tissues were harvested at 1.5,3 months after bilateral common carotid artery liga-tion respectively. The endothelial cells of basilar artery bifurcation were examined morphologically by using electron microscopy and immunohistochemically by using antibodies against β-catenin. Results On the day 0,1,4,7,14,28,35,42,49,56,70, and 84 after the first sugery, it was found that the basilar artery blood flow in experimental group was significantly increased as compared with sham control group. In experimental group,after 1.5 months,the β-catenin was mainly expressed on the nuclei,and after 3 months,the early stage of aneurysm formation on the basilar artery bifurcation appeared, but no expression of β-catenin was detected. Under the scanning electron microscopy, the endothelial cells were changed to column aspect from fusiform at 1.5 months, and there were cracks between endothelial cells and endothelial cell nucleus mar-gination. Conclusion By observing the endothelial cells changes at basilar artery bifurcation in response to sustained high blood flow in this rabbit model,it was suggested that the impairment of endothelial cells may initiate aneurysm formation.