中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
2期
161-165
,共5页
李宜儒%姚卫锋%展小飞%王敬%盛彩虹%李艳飞%冯斌%刘原君%齐蔓莉%刘全忠
李宜儒%姚衛鋒%展小飛%王敬%盛綵虹%李豔飛%馮斌%劉原君%齊蔓莉%劉全忠
리의유%요위봉%전소비%왕경%성채홍%리염비%풍빈%류원군%제만리%류전충
沙眼衣原体%重组主要外膜蛋白%疫苗%交叉免疫%恒河猴
沙眼衣原體%重組主要外膜蛋白%疫苗%交扠免疫%恆河猴
사안의원체%중조주요외막단백%역묘%교차면역%항하후
Chlamydia trachomatis%Recombinant major outer membrane protein%Vaccine%Immune response%Rhesus monkey
目的 探讨E型重组主要外膜蛋白(rMOMP)对恒河猴诱导产生的衣原体交叉免疫应答效应.方法 恒河猴分3组,每组2只,分别为佐剂蛋白组、佐剂组、对照组.于第0、2、4周双侧肱三头肌注射.末次免疫后两周,ELISA检测恒河猴血清中沙眼衣原体特异性IgG抗体和细胞因子IFN-γ,MTT法检测恒河猴淋巴细胞特异性增殖反应,观察恒河猴的迟发型超敏反应,以及恒河猴的血清抗体中和试验.结果 佐剂蛋白组产生了较强的抗rMOMP反应和高水平细胞因子.淋巴细胞特异性增殖反应、迟发型超敏反应明显强于对照组,抗体中和试验蛋白佐剂组血清能抑制D/E/H/L2型沙眼衣原体生长.结论 沙眼衣原体rMOMP能刺激恒河猴产生有效的交叉免疫.
目的 探討E型重組主要外膜蛋白(rMOMP)對恆河猴誘導產生的衣原體交扠免疫應答效應.方法 恆河猴分3組,每組2隻,分彆為佐劑蛋白組、佐劑組、對照組.于第0、2、4週雙側肱三頭肌註射.末次免疫後兩週,ELISA檢測恆河猴血清中沙眼衣原體特異性IgG抗體和細胞因子IFN-γ,MTT法檢測恆河猴淋巴細胞特異性增殖反應,觀察恆河猴的遲髮型超敏反應,以及恆河猴的血清抗體中和試驗.結果 佐劑蛋白組產生瞭較彊的抗rMOMP反應和高水平細胞因子.淋巴細胞特異性增殖反應、遲髮型超敏反應明顯彊于對照組,抗體中和試驗蛋白佐劑組血清能抑製D/E/H/L2型沙眼衣原體生長.結論 沙眼衣原體rMOMP能刺激恆河猴產生有效的交扠免疫.
목적 탐토E형중조주요외막단백(rMOMP)대항하후유도산생적의원체교차면역응답효응.방법 항하후분3조,매조2지,분별위좌제단백조、좌제조、대조조.우제0、2、4주쌍측굉삼두기주사.말차면역후량주,ELISA검측항하후혈청중사안의원체특이성IgG항체화세포인자IFN-γ,MTT법검측항하후림파세포특이성증식반응,관찰항하후적지발형초민반응,이급항하후적혈청항체중화시험.결과 좌제단백조산생료교강적항rMOMP반응화고수평세포인자.림파세포특이성증식반응、지발형초민반응명현강우대조조,항체중화시험단백좌제조혈청능억제D/E/H/L2형사안의원체생장.결론 사안의원체rMOMP능자격항하후산생유효적교차면역.
Objective To test cross immune responses induced in rhesus monkeys immunized with the recombinant major outer membrane protein(rMOMP).Methods Six rhesus monkeys were divided into three groups:the group vaccinated with purified rMOMP and Freund's adjutants,the group vaccinated with Freund's adjutants only and the control group vaccinated with PBS.All of the rhesus monkeys vaccinated intramuscularly at 0,2,4 weeks.Two weeks after the last time,The IFN-γand Chlamydia-specific antibody titers in sera,which were determined by ELISA,lymphocyte proliferation assay were performed by MTT,and observ the delayed hypersensitivity and in vitro neutralization assays.Results The result of the monkeys immunized with rMOMP and Freund's adjuvant:the specific immune responses can be observed.The in vitro neutralization and lymphocyte proliferation assays were observed better in the same group.Conclusion After being vaccinated with rMOMP,the monkeys can develop strong and effective Chlamydia-specific cross immune responses.
