CAJ | 학술논문

目的观察靶向封闭CapG基因对胰腺癌细胞株BxPC3运动侵袭能力的影响.方法设计并体外合成靶向CapG的小干扰RNA,将其转染到BxPC3细胞中,蛋白质印迹法检测转染前后细胞中CapG蛋白的表达,采用Transwell小室检测转染前后细胞运动侵袭能力的改变.结果 CapG siRNA能有效降低BxPC3细胞中CapG的表达,CapG在蛋白质水平的表达抑制率达80%左右.抑制BxPC3细胞中CapG的表达后,BxPC3细胞的运动侵袭能力明显下降,穿过人工基底膜的细胞数显著低于阴性对照组和空白对照组 (P<0 05).结论封闭CapG表达能明显抑制胰腺癌细胞BxPC3在体外的运动侵袭能力.
목적 관찰파향봉폐CapG기인대이선암세포주BxPC3운동침습능력적영향.방법 설계병체외합성파향CapG적소간우RNA,장기전염도BxPC3세포중,단백질인적법검측전염전후세포중CapG단백적표체,채용Transwell소실검측전염전후세포운동침습능력적개변.결과 CapG siRNA능유효강저BxPC3세포중CapG적표체,CapG재단백질수평적표체억제솔체80%좌우.억제BxPC3세포중CapG적표체후,BxPC3세포적운동침습능력명현하강,천과인공기저막적세포수현저저우음성대조조화공백대조조 (P<0 05).결론 봉폐CapG표체능명현억제이선암세포BxPC3재체외적운동침습능력.
Objective To investigate the effect of silenced CapG on migration and invasion of pancreatic cancer cell line BxPC3.Methods CapG small interfering RNA(siRNA) was designed and transfected into pancreatic cancer cell line BxPC3. The expression of CapG protein in BxPC3 was detected by western blot. The capability of moving and invasion of cancer cells were tested by Transwell.Results CapG protein expression was suppressed after CapG siRNA transfection. The migrational and invasional ability of BxPC3 cells transfected with CapG siRNA was significantly decreased compared with untransfected control cells (P<0 05). Conclusions Deletion of CapG gene can significantly inhibit the migration and invasion of BxPC3 cells in vitro.