天然产物研究与开发
天然產物研究與開髮
천연산물연구여개발
NATURAL PRODUCT RESEARCH AND DEVELOPMENT
2007年
1期
29-34
,共6页
穿山龙%多糖%纯化%抗氧化活性
穿山龍%多糖%純化%抗氧化活性
천산룡%다당%순화%항양화활성
Dioscorea nipponica Makino%polysaccharide%purification%antioxidant activities
采用热水煮提法和乙醇沉淀法从穿山龙中提取出水溶性粗多糖,再经DEAE-Sephrose CL-6B和Sephadex G-100柱层析分离纯化得到2个均多糖组分DMA和DMB.利用超氧阴离子实验,羟基阴离子实验和邻苯三酚自氧化实验研究DMA和DMB的抗氧化活性,结果表明DMB有较强的抗氧化活性,应该被作为潜在的抗氧化剂开发利用.
採用熱水煮提法和乙醇沉澱法從穿山龍中提取齣水溶性粗多糖,再經DEAE-Sephrose CL-6B和Sephadex G-100柱層析分離純化得到2箇均多糖組分DMA和DMB.利用超氧陰離子實驗,羥基陰離子實驗和鄰苯三酚自氧化實驗研究DMA和DMB的抗氧化活性,結果錶明DMB有較彊的抗氧化活性,應該被作為潛在的抗氧化劑開髮利用.
채용열수자제법화을순침정법종천산룡중제취출수용성조다당,재경DEAE-Sephrose CL-6B화Sephadex G-100주층석분리순화득도2개균다당조분DMA화DMB.이용초양음리자실험,간기음리자실험화린분삼분자양화실험연구DMA화DMB적항양화활성,결과표명DMB유교강적항양화활성,응해피작위잠재적항양화제개발이용.
The water-soluble crude polysaccharide,obtained from the Dioscorea nipponica Makino by boiling-water extraction and ethanol precipitation, was fractionated by DEAE-Sepharose CL-6B column chromatography and purified by Sephadex G-100,giving two polysaccharide fractions termed DMA and DMB. On the basis of superoxide radical assay,hydroxyl radical assay and self-oxidation of 1,2,3-phentriol assay, their antioxidant activities were investigated. Among them, DMB had the higher antioxidant activities and should be explored as a novel potential antioxidant agent.
