CAJ | 학술논문

云南金钱槭(Dipteronia dyeriana)是我国特有的国家Ⅱ级重点保护珍稀濒危植物,仅分布于云南的文山、屏边和蒙自三县交界地区.采用水平切片淀粉凝胶电泳等位酶分析方法,对采自三个县的天然居群的63个样品进行了遗传多样性检测.8个酶系统的13个等位基因位点分析表明,云南金钱槭的遗传多样性水平低,其平均多态位点百分率P=15.4%,每位点平均等位基因数A=1.2,平均预期杂合度H_e=0.064,各居群都偏离Hardy-Weinberg平衡,杂合子过量,居群间的基因分化系数G_(ST)=0.099,遗传变异主要发生在居群内(90.1%),居群间分化较小,居群间遗传一致度较高(I＝0.985～1),具有一定的基因流(Nm＝2.669).不加权对平均法(UPGMA)聚类可将4个居群分为两支,水头箐居群和马家寨居群亲缘关系最近,聚类后与黑洞居群形成一支再和中槽子居群聚类,这与其地理分布格局大致吻合.据此提出了关于云南金钱槭保育策略的建议.
운남금전축(Dipteronia dyeriana)시아국특유적국가Ⅱ급중점보호진희빈위식물,부분포우운남적문산、병변화몽자삼현교계지구.채용수평절편정분응효전영등위매분석방법,대채자삼개현적천연거군적63개양품진행료유전다양성검측.8개매계통적13개등위기인위점분석표명,운남금전축적유전다양성수평저,기평균다태위점백분솔P=15.4%,매위점평균등위기인수A=1.2,평균예기잡합도H_e=0.064,각거군도편리Hardy-Weinberg평형,잡합자과량,거군간적기인분화계수G_(ST)=0.099,유전변이주요발생재거군내(90.1%),거군간분화교소,거군간유전일치도교고(I＝0.985～1),구유일정적기인류(Nm＝2.669).불가권대평균법(UPGMA)취류가장4개거군분위량지,수두정거군화마가채거군친연관계최근,취류후여흑동거군형성일지재화중조자거군취류,저여기지리분포격국대치문합.거차제출료관우운남금전축보육책략적건의.
Dipteronia dyeriana Henry is a rare and endangered species restricted in a small adjacent corner of Wenshan,Pingbian and Mengzi counties of Yunnan,southwest China.The genetic diversity of four natural populations was estimated using allozyme markers.Thirteen loci of eight enzyme systems were scored for the analysis of population genetic diversity and structure.A low genetic diversity was observed in this rare species,with a mean percentage of polymorphic loci P=15.4%,an average number of alleles per locus A=1.2,and an average of expected heterozygosity H_e=0.064.All populations deviated significantly from the Hardy-Weinberg equilibrium.Very slight population differentiation was detected (G_(ST)=0.099).Nei's genetic identity ranged from 0.985 to 1.Moderate gene flow was inferred(Nm=2.669).According to the Unweighted Pair Group Method with Arithmetic（UPGMA）cluster analysis based on the genetic distance,Mengzi and Majiazhai populations are clustered in one clade and its genetic distances are positively related to the geographic disance.For conservation purpose,we strongly suggest strict protection of the natural populations of the species should be conducted,and ex situ conservation could be an effective supplement.