CAJ | 학술논문

[目的]探讨磷酸二酯酶3（PDE3）特异性抑制剂Milrinone与亮甲酚蓝染色液（BCB）相结合在绵羊卵母细胞体外培养中的应用。[方法]比较了BCB对绵羊卵母细胞的筛选与传统的形态分级之间的差异,以及对胚胎后期发育的影响;研究了Milrinone对绵羊卵母细胞的最佳抑制时间,并用于BCB-卵母细胞体外培养。[结果]A、B级卵母细胞中BCB＋卵母细胞的比例为64.42%,极显著高于C级卵母细胞的17.00%; BCB＋卵母细胞的成熟率（86.16%）、卵裂率（85.29%）、囊胚率（34.40%）分别极显著高于BCB-卵母细胞（50.94%、36.19%和6.73%）;6 h是Milrinone对绵羊卵母细胞的最佳抑制时间,该试验组体外培养效率显著高于其他组;Milrinone对BCB-卵母细胞抑制培养6 h,极显著提高了体外胚胎发育率。[结论]为提高绵羊卵母细胞体外培养效率提供了依据。
[목적]탐토린산이지매3（PDE3）특이성억제제Milrinone여량갑분람염색액（BCB）상결합재면양란모세포체외배양중적응용。[방법]비교료BCB대면양란모세포적사선여전통적형태분급지간적차이,이급대배태후기발육적영향;연구료Milrinone대면양란모세포적최가억제시간,병용우BCB-란모세포체외배양。[결과]A、B급란모세포중BCB＋란모세포적비례위64.42%,겁현저고우C급란모세포적17.00%; BCB＋란모세포적성숙솔（86.16%）、란렬솔（85.29%）、낭배솔（34.40%）분별겁현저고우BCB-란모세포（50.94%、36.19%화6.73%）;6 h시Milrinone대면양란모세포적최가억제시간,해시험조체외배양효솔현저고우기타조;Milrinone대BCB-란모세포억제배양6 h,겁현저제고료체외배태발육솔。[결론]위제고면양란모세포체외배양효솔제공료의거。
[Objective] The aim of this study was to increase the viability of sheep oocytes in vitro by using phosphodiesterase type 3（PDE 3） inhibitor milrinone combined with brilliant cresyl blue（BCB） staining.[Method] The differences between BCB tested and morphologically selected oocytes,as well as the effect of them on embryo development were compared;and then suitable inhibitive time of milrinone to sheep oocytes in vitro was studied and used in BCB-oocytes for in vitro embryo production（IVEP）.[Result] The BCB＋ oocytes percentage in A-and B-level sheep oocytes was 64.42%,which was extremely significantly higher than that in C-level（17.0%）.The maturing rate,cleavage rate and blastocyst rate of BCB＋ oocytes（86.16%,85.29% and 34.40%） of was significantly higher than those of BCB-oocytes（50.94%,36.19% and 6.73%）.The best time for PDE 3 inhibitor delaying the sheep oocyte mature in vitro was 6 h.In addition,the rate of embryo development in vitro could be significantly increased by inhibiting the BCB-oocytes for 6 h with Milrinone.[Conclusion] The study will provide reference for improving the efficiency of sheep oocytes culture in vitro.