CAJ | 학술논문

目的通过二血管阻断加低血压法建立大鼠全脑缺血再灌注模型,探讨全脑缺血再灌注大鼠大脑组织结构变化,为脑复苏中的进一步神经保护作用研究提供条件.方法 30只SD大鼠采用二血管阻断加低血压法制作大鼠全脑缺血再灌注模型;实验结束后,计算成功例数和死亡例数.开颅取大脑组织进行形态学观察.结果 30只大鼠中,26只造模成功,成功率86.67%.全脑缺血再灌注大鼠大脑出现大量神经元坏死、胞浆浓缩、核固缩现象.结论二血管阻断加低血压法成模效果稳定,死亡率较低.能较好地建立大鼠全脑缺血冉灌注模型,可为脑复苏的的深入研究提供良好实验基础.
목적 통과이혈관조단가저혈압법건립대서전뇌결혈재관주모형,탐토전뇌결혈재관주대서대뇌조직결구변화,위뇌복소중적진일보신경보호작용연구제공조건.방법 30지SD대서채용이혈관조단가저혈압법제작대서전뇌결혈재관주모형;실험결속후,계산성공례수화사망례수.개로취대뇌조직진행형태학관찰.결과 30지대서중,26지조모성공,성공솔86.67%.전뇌결혈재관주대서대뇌출현대량신경원배사、포장농축、핵고축현상.결론 이혈관조단가저혈압법성모효과은정,사망솔교저.능교호지건립대서전뇌결혈염관주모형,가위뇌복소적적심입연구제공량호실험기출.
Objective Through two vessel occlusion add hypotension to establish the global cerebral is-chemia/reperfusion model, discusses the global cerebral structure change in rats. Methods Global cerebral is-chemia-reperfusion model was established by two-vessel occlusion plus hypotension in 30 SD rats. Successful cases and death cases were calculated and cerebral morphological changes were observed. Results Of 30 rats, 26 (86. 67%) were successful, and morphological observation showed obvious neurons lesions including neurons necrosis, concentrated cytoplasma and pyknosis. Conclusion The method of two vessel occlusion plus hypotension could establish global cerebral ischemia/reperfusion model in rat. It was stability, typical and lower mortality.