中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2012年
3期
272-275,278
,共5页
重组人促红细胞生成素%高浓度氧%肺损伤%大鼠,新生
重組人促紅細胞生成素%高濃度氧%肺損傷%大鼠,新生
중조인촉홍세포생성소%고농도양%폐손상%대서,신생
Recombinant human erythropoietin%Hyperoxia%Lung injury%Rat,newborn
目的 探讨不同剂量重组人促红细胞生成素(recombinant humanerythropoietin,rhEPO)作为血管生长样因子对新生大鼠高氧肺损伤的血管保护作用.方法 新生Sprague-Dawley大鼠60只,分为空气组、高氧组(持续吸入95％的高浓度氧)、高氧+大剂量rhEPO组(于高氧暴露前1h及暴露3d后,腹腔注射rhEPO5000 U/kg)及高氧+小剂量rhEPO组(时间点同前,腹腔注射rhEPO800 U/kg),每组15只.而空气组和高氧组分别于同一时间点腹腔注射等量生理盐水.高氧暴露6d时,观察各组大鼠存活率变化,免疫组织化学法检测肺组织血管内皮标志CD31及肺血管内皮细胞生长因子(Vascular endothelial growth factor,VEGF)表达的变化.结果 高氧暴露6d后,与高氧组相比,高氧+大剂量rhEPO组大鼠存活率显著提高[86.7％ (13/15) vs 60.0％(9/15)]；肺组织CD31阳性面积比[(38.69±1.69)％ vs (33.57±4.12)％,P＜0.05]和VEGF的表达(124.4296±7.282 3 vs 114.205 9±8.345 7,P＜0.05)明显增高；而高氧+小剂量rhEPO组肺组织CD31阳性面积比[(36.34±1.89)％]及VEGF的表达(115.4296±6.7199)无明显改善,差异无统计学意义(P＞0.05).结论 大剂量rhEPO(5000 U/kg)可以促进肺血管的发育和修复,对新生鼠高氧肺损伤有血管保护作用,而800 U/kg rhEPO无明显的高氧肺损伤血管保护作用.
目的 探討不同劑量重組人促紅細胞生成素(recombinant humanerythropoietin,rhEPO)作為血管生長樣因子對新生大鼠高氧肺損傷的血管保護作用.方法 新生Sprague-Dawley大鼠60隻,分為空氣組、高氧組(持續吸入95％的高濃度氧)、高氧+大劑量rhEPO組(于高氧暴露前1h及暴露3d後,腹腔註射rhEPO5000 U/kg)及高氧+小劑量rhEPO組(時間點同前,腹腔註射rhEPO800 U/kg),每組15隻.而空氣組和高氧組分彆于同一時間點腹腔註射等量生理鹽水.高氧暴露6d時,觀察各組大鼠存活率變化,免疫組織化學法檢測肺組織血管內皮標誌CD31及肺血管內皮細胞生長因子(Vascular endothelial growth factor,VEGF)錶達的變化.結果 高氧暴露6d後,與高氧組相比,高氧+大劑量rhEPO組大鼠存活率顯著提高[86.7％ (13/15) vs 60.0％(9/15)]；肺組織CD31暘性麵積比[(38.69±1.69)％ vs (33.57±4.12)％,P＜0.05]和VEGF的錶達(124.4296±7.282 3 vs 114.205 9±8.345 7,P＜0.05)明顯增高；而高氧+小劑量rhEPO組肺組織CD31暘性麵積比[(36.34±1.89)％]及VEGF的錶達(115.4296±6.7199)無明顯改善,差異無統計學意義(P＞0.05).結論 大劑量rhEPO(5000 U/kg)可以促進肺血管的髮育和脩複,對新生鼠高氧肺損傷有血管保護作用,而800 U/kg rhEPO無明顯的高氧肺損傷血管保護作用.
목적 탐토불동제량중조인촉홍세포생성소(recombinant humanerythropoietin,rhEPO)작위혈관생장양인자대신생대서고양폐손상적혈관보호작용.방법 신생Sprague-Dawley대서60지,분위공기조、고양조(지속흡입95％적고농도양)、고양+대제량rhEPO조(우고양폭로전1h급폭로3d후,복강주사rhEPO5000 U/kg)급고양+소제량rhEPO조(시간점동전,복강주사rhEPO800 U/kg),매조15지.이공기조화고양조분별우동일시간점복강주사등량생리염수.고양폭로6d시,관찰각조대서존활솔변화,면역조직화학법검측폐조직혈관내피표지CD31급폐혈관내피세포생장인자(Vascular endothelial growth factor,VEGF)표체적변화.결과 고양폭로6d후,여고양조상비,고양+대제량rhEPO조대서존활솔현저제고[86.7％ (13/15) vs 60.0％(9/15)]；폐조직CD31양성면적비[(38.69±1.69)％ vs (33.57±4.12)％,P＜0.05]화VEGF적표체(124.4296±7.282 3 vs 114.205 9±8.345 7,P＜0.05)명현증고；이고양+소제량rhEPO조폐조직CD31양성면적비[(36.34±1.89)％]급VEGF적표체(115.4296±6.7199)무명현개선,차이무통계학의의(P＞0.05).결론 대제량rhEPO(5000 U/kg)가이촉진폐혈관적발육화수복,대신생서고양폐손상유혈관보호작용,이800 U/kg rhEPO무명현적고양폐손상혈관보호작용.
Objective To investigate the effect of different dosage recombinant human erythropoietin(rhEPO),an angiogenesis-like factor,on pulmonary angiogenesis exposed to hyperoxia in newborn rats.Methods Sixty Sprague-Dawley newborn rats were randomly divided into four groups:air group (room air exposure,n =15 ),hyperoxia group ( exposed to 95％ oxygen,n =15 ),hyperoxia + large dosage rhEPO group (received rhEPO 5000 U/kg,intraperitoneally on 1 hour before and 3 days after exposed to hyperoxia,n =15) and hyperoxia + small dosage rhEPO group (received rhEPO 800U/kg,the same time points,n =15 ).The isodose of saline were given intraperitoneally on the same time points in the air group and the hyperoxia group.After 6 d of exposure,the survival rate was compared,CD31 and vascular endothelial growth factor (VEGF) were measured by immunohistochemistry to assess hyperoxia-induced changes in lung morphology.Results After 6d of exposure,hyperoxia + large dosage rhEPO group prolonged the survival rate in comparison with the hyperoxia group [ 86.7 ％ ( 13/15 ) vs 60.0 ％ ( 9/15 ) ].The expression of lung CD31 [ ( 38.69 ±1.69)％ vs (33.57±4.12)％,P＜0.05] and VEGF (124.4296±7.2823 vs 114.2059 ±-8.345 7,P＜0.05) in newborn rats treated with large dosage of rhEPO was significantly higher than those in hyperoxia group.While there was no significant difference of CD31 [ ( 36.34 ± 1.89 ) ％ ] and VEGF( 115.429 6 ± 6.719 9) in small dosage rhEPO group compared with the hyperoxia group (P＞0.05 ).Conclusion Instead of treatment with small dosage rhEPO (800 U/kg),large dosage rhEPO (5000 U/kg) may have important protective effects on pulmonary angiogenesis in hyperoxia-induced lung injury of newborn rats.
