CAJ | 학술논문

目的研究生物钟基因Per2(Circadian gene Per2)和血管内皮生长因子(VEGF)在非小细胞肺癌中的表达,探讨其临床意义.方法采用免疫组织化学染色法检测60例非小细胞肺癌组织和20例正常肺组织中Per2和VEGF的表达,分析其表达与临床病理特征的关系.结果非小细胞肺癌组织中Per2阳性表达率为71.7％,低于正常肺组织中的95.0％ (x2 =4.683,P＜0.05),而VEGF的阳性表达率为58.3％,高于正常肺组织中的15.0％(x2=11.295,P＜0.01).非小细胞肺癌中Per2的表达缺失与分化程度及TMN分期相关(x2 =4.413,6.179,均P＜0.05),而VEGF的阳性表达与分化程度及淋巴结转移相关(x2=6.524,P＜0.05).两者在非小细胞肺癌中的表达呈负相关(r=-0.381,P＜0.01).结论 Per2在非小细胞肺癌中表达降低,而VEGF则呈高表达,两者在非小细胞肺癌的发生、发展中可能起相反的作用,影响其预后.
목적 연구생물종기인Per2(Circadian gene Per2)화혈관내피생장인자(VEGF)재비소세포폐암중적표체,탐토기림상의의.방법 채용면역조직화학염색법검측60례비소세포폐암조직화20례정상폐조직중Per2화VEGF적표체,분석기표체여림상병리특정적관계.결과 비소세포폐암조직중Per2양성표체솔위71.7％,저우정상폐조직중적95.0％ (x2 =4.683,P＜0.05),이VEGF적양성표체솔위58.3％,고우정상폐조직중적15.0％(x2=11.295,P＜0.01).비소세포폐암중Per2적표체결실여분화정도급TMN분기상관(x2 =4.413,6.179,균P＜0.05),이VEGF적양성표체여분화정도급림파결전이상관(x2=6.524,P＜0.05).량자재비소세포폐암중적표체정부상관(r=-0.381,P＜0.01).결론 Per2재비소세포폐암중표체강저,이VEGF칙정고표체,량자재비소세포폐암적발생、발전중가능기상반적작용,영향기예후.
Objective To detect the expression of Per2 and VEGF in NSCLC tissue,and analyze its clinical significance.Methods The expression of Per2 and VEGF was measured in 60 NSCLC and 20 normal lung tissue by immunohistochemistry assay,then the relation with clinicopathological parameters was analyzed.Results The positive expression rate of Per2 in NSCLC and in normal lung tissue were 71.7％ and 95.0％ ( x2 =4.683,P ＜0.05 ),while the positive expression rate of VEGF were 58.3％ and 15％ (x2 =11.295,P ＜ 0.01 ).The negative expression of Per2 in NSCLC was correlated with pathological differentiation or TNM stage( x2 =4.413,6.179,all P ＜0.05),while the positive expression of VEGF in NSCLC was correlated with pathological differentiation or lymphatic metastasis (x2 =6.524,P ＜ 0.05 ).The expression of Per2 was negatively correlated with the expression of VEGF in NSCLC (r =-0.381,P ＜ 0.01 ).Conclusion The overexpression of VEGF in NSCLC may contribute to invasion and metastasis,while Per2 may be a beneficial prognosis factor in patients with NSCLC.