CAJ | 학술논문

目的通过对相同照射剂量、不同照射时间的放射对小鼠Lewis肺癌肿瘤生长延迟时间、小鼠生存时间的研究,探讨分次照射不同间隔时间对肿瘤的放射效应的影响.方法将移植Lewis肺癌种鼠的肿瘤组织制备成1 × 107个/ml肿瘤细胞悬液,选掸48只8-10周雄性C57BL/6J小鼠右后肢外侧小腿腓肠肌处接种0.1 ml,建市移植瘤小鼠模型.肿瘤直径达0.8-1.0 cm时随机分成6个组:空白对照组、18 Gy单次照射组,9 Gy 2次每次间隔30 min组,2.57 Gy 7次每次间隔5 min组、9 Gy 2次每次间隔60 min组、2.57 Gy 7次每次问隔10 min组.隔日测量并记录肿瘤长短直径,观察肿瘤生长曲线、生长延缓时间及小鼠生存时间.结果不同间隔时间各组生长延缓时间均<18 Gy单次照射组.照射总间隔时间30 min与60 min相比,小鼠肿瘤的生长变缓;而放射总间隔时间相同的各组,小鼠肿瘤生长延缓相似.与18 Gy单次照射组比较,放射总间隔时间延长缩短了小鼠生存时间;放射间隔时间30min和60min对小鼠肿瘤牛长影响相似.结论小鼠Lewis肺癌模型中,相间照射剂量、放射总间隔时间延长降低了放射疗效,表现为缩短了小鼠肿瘤牛长延缓时间及总生存时间.对小鼠肿瘤生长影响程度与放射间隔时间的长短有关,间隔时间越长对小鼠肿瘤生长影响越大,但间隔30、60 min之间对小鼠生存时间影响差别不大.
목적 통과대상동조사제량、불동조사시간적방사대소서Lewis폐암종류생장연지시간、소서생존시간적연구,탐토분차조사불동간격시간대종류적방사효응적영향.방법 장이식Lewis폐암충서적종류조직제비성1 × 107개/ml종류세포현액,선탄48지8-10주웅성C57BL/6J소서우후지외측소퇴비장기처접충0.1 ml,건시이식류소서모형.종류직경체0.8-1.0 cm시수궤분성6개조:공백대조조、18 Gy단차조사조,9 Gy 2차매차간격30 min조,2.57 Gy 7차매차간격5 min조、9 Gy 2차매차간격60 min조、2.57 Gy 7차매차문격10 min조.격일측량병기록종류장단직경,관찰종류생장곡선、생장연완시간급소서생존시간.결과 불동간격시간각조생장연완시간균<18 Gy단차조사조.조사총간격시간30 min여60 min상비,소서종류적생장변완;이방사총간격시간상동적각조,소서종류생장연완상사.여18 Gy단차조사조비교,방사총간격시간연장축단료소서생존시간;방사간격시간30min화60min대소서종류우장영향상사.결론 소서Lewis폐암모형중,상간조사제량、방사총간격시간연장강저료방사료효,표현위축단료소서종류우장연완시간급총생존시간.대소서종류생장영향정도여방사간격시간적장단유관,간격시간월장대소서종류생장영향월대,단간격30、60 min지간대소서생존시간영향차별불대.
Objective To study the effect of different fraction interval with same total radiation dose on tumor growth delay and survival in C57BL mice implanted with lewis lung cancer,and to determine whether prolonged fraction interval will decrease the tumor response to radiation. Methods Forty-eight mice were implanted with lewis lung cancer in the back legs.When the diameter of transplanted tumor reached 0.8 to 1 cm,the mice were randomized into 6 groups:normal control group,single fraction of 18 Gy group,18 Gy in 2 fractions of 9 Gy at 30 min interval group,18 Gy in 7 fractions of 2.57 Gy at 5 min inter val group,18 Gy in 2 fractions of 9 Gy at 60 min interval group and 18 Gy in 7 fractions of 2.57 Gy at 10 rain interval group.The maximal and minimal diameters of the tumor were measured and record every other day to study the tumor growth tendency,the tumor growth delay and the mice survival time. Results The tumor growth delay of groups at prolonged fraction interval was shorter than the group with single fraction of 18 Gy (P < 0.05).The tumor growth delay of groups at fraction interval of 30 rain was longer than that of groups at interval of 60 rain (P < 0.05).There was no significant difference of the tumor growth delay be tween the groups with same delivery time (P >0.05).The mice survival time of the groups with prolonged fraction interval was shortened when omparing to the group with single fraction of 18 Gy.While the difference was not significant between the groups at fraction interval of 30 min and 60 min. Conclusions The pro longed fraction interval but same total radiation dose shortens the tumor growth delay and survival time in the mice implanted with Lewis lung cancer.The longer fraction interval impairs the tumor control more signifi candy.However the difference of the effect on mice survival time is not significant between the groups at fraction interval of 30 min and 60 min.