中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2011年
2期
134-137
,共4页
倪冠英%武宁%郭海卓%金顺子
倪冠英%武寧%郭海卓%金順子
예관영%무저%곽해탁%금순자
电离辐射%Jurkat细胞%P21蛋白%p21基因%胸腺细胞%脾细胞
電離輻射%Jurkat細胞%P21蛋白%p21基因%胸腺細胞%脾細胞
전리복사%Jurkat세포%P21단백%p21기인%흉선세포%비세포
Ionizing radiation%Jurkat cell line%P21 protein%p21 gene%Thymocyte%Splenocyte
目的 探讨电离辐射对Jurkat细胞P21蛋白和ICR小鼠胸腺细胞p21基因表达的影响.方法 采用流式细胞术(FCM),检测0、0.5、1.0、2.0、4.0及6.0 Gy X射线照射后Jurkat细胞中P21蛋白表达的变化.采用实时定量PCR技术,分别检测0、0.5、1.0、2.0、4.0及6.0 Gy X射线照射后4和24 hd'鼠胸腺及脾细胞中p21基因表达的变化.结果 不同剂量X射线照射后12和24 h,Jurkat细胞中P21蛋白表达在0.5～4.0 Gy范围内均随剂量的增大而升高(t=-24.23～-3.96,P<0.05),6 Gy时均出现表达下降(t=-11.19、-14.50,P<0.05);与假照射组相比,在0～6.0 Gy照射后4和24 h,小鼠胸腺及脾细胞中p21基因的相对表达量均随剂量增大逐渐增加(t=-29.96～8.80,P<0.05);并于6.0 Gy时达最高(t=-11.84～-3.42,P<0.05),仅胸腺细胞1 Gy照射后4 h除外(t=-3.42,P>0.05).结论 x射线能诱导P21蛋白及基因表达增加,并在一定剂量范围内存在良好的剂量-效应关系.
目的 探討電離輻射對Jurkat細胞P21蛋白和ICR小鼠胸腺細胞p21基因錶達的影響.方法 採用流式細胞術(FCM),檢測0、0.5、1.0、2.0、4.0及6.0 Gy X射線照射後Jurkat細胞中P21蛋白錶達的變化.採用實時定量PCR技術,分彆檢測0、0.5、1.0、2.0、4.0及6.0 Gy X射線照射後4和24 hd'鼠胸腺及脾細胞中p21基因錶達的變化.結果 不同劑量X射線照射後12和24 h,Jurkat細胞中P21蛋白錶達在0.5～4.0 Gy範圍內均隨劑量的增大而升高(t=-24.23～-3.96,P<0.05),6 Gy時均齣現錶達下降(t=-11.19、-14.50,P<0.05);與假照射組相比,在0～6.0 Gy照射後4和24 h,小鼠胸腺及脾細胞中p21基因的相對錶達量均隨劑量增大逐漸增加(t=-29.96～8.80,P<0.05);併于6.0 Gy時達最高(t=-11.84～-3.42,P<0.05),僅胸腺細胞1 Gy照射後4 h除外(t=-3.42,P>0.05).結論 x射線能誘導P21蛋白及基因錶達增加,併在一定劑量範圍內存在良好的劑量-效應關繫.
목적 탐토전리복사대Jurkat세포P21단백화ICR소서흉선세포p21기인표체적영향.방법 채용류식세포술(FCM),검측0、0.5、1.0、2.0、4.0급6.0 Gy X사선조사후Jurkat세포중P21단백표체적변화.채용실시정량PCR기술,분별검측0、0.5、1.0、2.0、4.0급6.0 Gy X사선조사후4화24 hd'서흉선급비세포중p21기인표체적변화.결과 불동제량X사선조사후12화24 h,Jurkat세포중P21단백표체재0.5～4.0 Gy범위내균수제량적증대이승고(t=-24.23～-3.96,P<0.05),6 Gy시균출현표체하강(t=-11.19、-14.50,P<0.05);여가조사조상비,재0～6.0 Gy조사후4화24 h,소서흉선급비세포중p21기인적상대표체량균수제량증대축점증가(t=-29.96～8.80,P<0.05);병우6.0 Gy시체최고(t=-11.84～-3.42,P<0.05),부흉선세포1 Gy조사후4 h제외(t=-3.42,P>0.05).결론 x사선능유도P21단백급기인표체증가,병재일정제량범위내존재량호적제량-효응관계.
Objective To investigate the effects of ionizing radiation on the expression of P21 protein in Jurkat cell line and p21 gene in thymocytes and splenocytes of mice.Methods Flow cytometry (FCM)was used to analyze the expression of P21 protein in Jurkat cells at 12 and 24 h after irradiation to 0,0.5,1.0,2.0,4.0,and 6.0 Gy.Real-time PCR was used to detect the expression of p21 gene in thymocytes and splenocytes of mice at4 and 24 h after irradiation to 0,0.5,1.0,2.0,4.0,and 6.0 Gy.Multi-staining was used to analyze the micronucleus rates of Rct in bone marrow.Results The expressions of P21 protein were increased in a dose-dependent manner during 0.5-4.0 Gy(t=-24.23--3.96,P<0.05),but decreased at 6.0 Gy at 12 and 24 h post-irradiation(t=-11.19,-14.50,P<0.05).The expressions of p2 1 gene in both thymocytes and splenocytes of mice were increased in dose-dependent manner in the range of 0-6.0 Gy(including 6.0 Gy)(t=-29.96-8.80,P<0.05),and reached to the peak at 6.0 Gy at 4 and 24 h post-irradiation(t=-11.84--3.42,P<0.05),except thymocytes at 4 h and 1.0 Gy post-irradiation(t=-3.42,P>0.05).Conclusions The expressions of P21 protein and p21 gene could be increased by X-ray irradiation.which shows good dosedependent manners in certain range of dose.
