中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2009年
14期
994-996
,共3页
张毅%夏育民%刘小明%车巍%熊腊元%徐世正
張毅%夏育民%劉小明%車巍%熊臘元%徐世正
장의%하육민%류소명%차외%웅석원%서세정
氨基酮戊酸%光化学疗法%温度%血红素%Hep-2细胞
氨基酮戊痠%光化學療法%溫度%血紅素%Hep-2細胞
안기동무산%광화학요법%온도%혈홍소%Hep-2세포
Aminolevulinic acid%Photochemotherapy%Temperature%Heme%Hep-2 cell
目的 探讨温度变化对δ-氨基酮戊酸(ALA)诱导的喉鳞癌系Hep-2细胞光动力学效应的影响.方法 将Hep-2细胞分成实验组(含2 mmol/L ALA)和对照组(未加ALA),在19～46℃时避光孵育.经激光照射诱导光动力反应之前,检测胞内原卟啉Ⅸ(PpⅨ)水平和细胞死亡率(坏死与凋亡);激光照射后,再次检测细胞死亡率.结果 照光前随着温度上升,实验组胞内PpⅨ水平由(0.25±0.06)μg/L提高至(1.07±0.11)μg/L,对照组胞内未能测出PpⅨ.照光前,在19～37℃区间,两组细胞的死亡率变化不明显;在37～46℃区间,实验组细胞死亡率由2.33%增加至38.99%,对照组由1.79%增加至36.66%,两组间死亡率差异无统计学意义(P>0.05).照光后:对照组的细胞死亡率变化与照光前相同,而实验组则随着温度增加而升高(31.11%～98.92%);在相同温度下实验组结果均高于对照组(P<0.05),且随着温度上升,两组差值由28.99%增大至59.26%.结论 适度升高培养温度可以增强ALA诱导的Hep-2细胞光动力学效应.
目的 探討溫度變化對δ-氨基酮戊痠(ALA)誘導的喉鱗癌繫Hep-2細胞光動力學效應的影響.方法 將Hep-2細胞分成實驗組(含2 mmol/L ALA)和對照組(未加ALA),在19～46℃時避光孵育.經激光照射誘導光動力反應之前,檢測胞內原卟啉Ⅸ(PpⅨ)水平和細胞死亡率(壞死與凋亡);激光照射後,再次檢測細胞死亡率.結果 照光前隨著溫度上升,實驗組胞內PpⅨ水平由(0.25±0.06)μg/L提高至(1.07±0.11)μg/L,對照組胞內未能測齣PpⅨ.照光前,在19～37℃區間,兩組細胞的死亡率變化不明顯;在37～46℃區間,實驗組細胞死亡率由2.33%增加至38.99%,對照組由1.79%增加至36.66%,兩組間死亡率差異無統計學意義(P>0.05).照光後:對照組的細胞死亡率變化與照光前相同,而實驗組則隨著溫度增加而升高(31.11%～98.92%);在相同溫度下實驗組結果均高于對照組(P<0.05),且隨著溫度上升,兩組差值由28.99%增大至59.26%.結論 適度升高培養溫度可以增彊ALA誘導的Hep-2細胞光動力學效應.
목적 탐토온도변화대δ-안기동무산(ALA)유도적후린암계Hep-2세포광동역학효응적영향.방법 장Hep-2세포분성실험조(함2 mmol/L ALA)화대조조(미가ALA),재19～46℃시피광부육.경격광조사유도광동력반응지전,검측포내원계람Ⅸ(PpⅨ)수평화세포사망솔(배사여조망);격광조사후,재차검측세포사망솔.결과 조광전수착온도상승,실험조포내PpⅨ수평유(0.25±0.06)μg/L제고지(1.07±0.11)μg/L,대조조포내미능측출PpⅨ.조광전,재19～37℃구간,량조세포적사망솔변화불명현;재37～46℃구간,실험조세포사망솔유2.33%증가지38.99%,대조조유1.79%증가지36.66%,량조간사망솔차이무통계학의의(P>0.05).조광후:대조조적세포사망솔변화여조광전상동,이실험조칙수착온도증가이승고(31.11%～98.92%);재상동온도하실험조결과균고우대조조(P<0.05),차수착온도상승,량조차치유28.99%증대지59.26%.결론 괄도승고배양온도가이증강ALA유도적Hep-2세포광동역학효응.
Objective To investigate effect of temperature on δ-aminolevulinic acid(AIA)induced photodynamic reaction in laryngeal squamous carcinoma cells.Methods Human laryngeal squamous carcinoma cells of the line Hep-2 cells were co-cultured with 2 retool/L ALA(Group A)or without ALA(Group B)at the culturing temperature 19-46℃.Three hours later celhlar protoporphyrin IX(PpⅨ)level was determined by hish performance liquid chromatography with fluorescent detection.Lager scanning confocal microscopy was used to observe the fluorescent strength in the Hep-2 cells.The ratio of cell death(including necrosis and apoptosis)was detected with flow cytometer before and after phtotedynamic reaction.Results In Group A the PpIX level at the temperature of 19℃Was(0.25±0.06)μg/L,and raised to(1.07±0.11)μg/L at 46℃.There Wag no cellular PpⅨdetected in Group B at any temperature.Before laser radiation the cell death ratios of both groups were the same at the temperature 19-37℃.and at the temperature 37-46℃.After laser radiation the cell death ratio of Group A raised from 31.11%t0 98.92%as the temperature went up steadily.but tIle results of Group B showed the sine curve as before laser radiation.At any same temperatures the cell death ratios of Group A were all significantly higher than those of Group B(all P<0.05),and as the temperature was elevated the difference between the 2 groups raised from 28.99%t0 59.26%.Conclusion Moderate higher temperature enhances the PpⅨproduction and photodynamic reaction in human laryngeal squamous carcinoma induced by ALA in vitro.
