CAJ | 학술논문

目的研究蛋白酶体β5亚基在人动脉粥样硬化斑块中表达水平的变化.方法采用免疫组织化学、Western印迹方法,检测16例颈动脉粥样硬化斑块与斑块旁内膜(病例组),以及6例相对正常的(对照组)外周动脉内膜组织中蛋白酶体β5亚基的表达.结果 (1)与对照相比,病例组高脂血症、糖尿病的比率较高,并多伴有吸烟史；(2)免疫组织化学分析显示,在平滑肌细胞的胞质及胞核中均存在蛋白酶体 β5亚基的阳性信号,且以在胞质表达为主；(3)蛋白酶体β5亚基在动脉硬化斑块中表达强度为(-～++),阳性为9/16例；斑块旁内膜中表达强度为(-～+++),阳性为11/16例；对照组中表达强度为(+～+++),阳性为6/6例.3组间蛋白酶体 β5亚基间阳性细胞的表达强度差异均有统计学意义(均P＜0.05).结论在动脉粥样硬化斑块中,蛋白酶体β5亚基蛋白质表达水平显著降低.
목적 연구단백매체β5아기재인동맥죽양경화반괴중표체수평적변화.방법 채용면역조직화학、Western인적방법,검측16례경동맥죽양경화반괴여반괴방내막(병례조),이급6례상대정상적(대조조)외주동맥내막조직중단백매체β5아기적표체.결과 (1)여대조상비,병례조고지혈증、당뇨병적비솔교고,병다반유흡연사；(2)면역조직화학분석현시,재평활기세포적포질급포핵중균존재단백매체 β5아기적양성신호,차이재포질표체위주；(3)단백매체β5아기재동맥경화반괴중표체강도위(-～++),양성위9/16례；반괴방내막중표체강도위(-～+++),양성위11/16례；대조조중표체강도위(+～+++),양성위6/6례.3조간단백매체 β5아기간양성세포적표체강도차이균유통계학의의(균P＜0.05).결론 재동맥죽양경화반괴중,단백매체β5아기단백질표체수평현저강저.
Objective To evaluate the expression of proteasome β5 subunit in human atherosclerotic plaque.Methods The specimens of carotid endarterectomy were collected from 16 carotid stenosis patients,For the case group,they were divided into 2 groups:plaque (group A) and arcas adjacent to plaque (group B).And carotid endarterium was obtained from 6 patients undergoing aortic replacement surgery.Relatively normal arterial intima ( subclavian artery & innominate artery) was selected as the control group (group C).Content of proteasome β5 subunit was evaluated by hematoxylin & eosin (HE) staining,immunohistochemistry and Western blot. Results (1)In patients with diabetes, hyperlipidemia and probable smoking,proteasome β5 subunit was enhanced in all groups; (2) Proteasome β5 subunit was demonstrated in both cytoplasm and nucleus of vascular smooth muscle cells,but mainly confined to cytoplasm; (3)The positive expression rate of β5 was 9/16 in group A,11/16 in group B and 6/6 in group C. There was significant divergence in the expression of β5 in different sample sub-groups:group A ( atherosclerotic plaque) ＜ group B ( areas adjacent to plaque) ＜ group C ( control group).Conclusion The exprcssion of β5 decrease markedly in human atherosclerotic plaques.