中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2010年
3期
162-165
,共4页
张薇%高峻%邹多武%许国铭
張薇%高峻%鄒多武%許國銘
장미%고준%추다무%허국명
内脏高敏感%匹维溴胺%蛋白酶激活受体2
內髒高敏感%匹維溴胺%蛋白酶激活受體2
내장고민감%필유추알%단백매격활수체2
Visceral hypersensitivity%Pinaverium bromide%Proteinase activated-receptor 2
目的 观察匹维溴胺对大鼠内脏高敏感形成及蛋白酶激活受体2(PAR2)表达的影响.方法 采用慢性避水应激法(WAS)构建内脏高敏感大鼠模型.Wistar大鼠随机分为假避水应激组、避水应激组和匹维溴胺治疗组(30 mg·kg~(-1)·d~(-1)),每组12只.观察每组大鼠应激时的排便颗粒数,并以结直肠扩张时的内脏运动反射(VMR)幅度值为指标观察内脏敏感性的变化.采用Western印迹和免疫荧光法检测结肠黏膜上皮PAR2的表达.结果 应激组大鼠排便颗粒数高于假避水应激组[(8.104±2.75)颗比(4.13±1.36)颗,P<0.001],匹维溴胺干预能减少应激时的排便颗粒数[(5.87±1.78)颗,P<0.001].避水应激后大鼠对60 mmHg(1 mmHg=0.133 kPa)压力扩张刺激的VMR幅度值(8.64±2.88)高于假避水应激组(3.805±1.04,P<0.001),匹维溴胺治疗可降低模型大鼠的VMR幅度值(6.09±0.77,P=0.02).与假避水应激组相比,避水应激上调结肠PAR2表达(相对灰度值分别为0.32±0.04和0.70±0.06,累积A值分别为77.16±10.74及279.085±100.91,P<0.001),匹维溴胺治疗抑制了PAR2的表达上调(相对灰度值0.53±0.06,累积A值180.59±25.75,P<0.05).结论 匹维溴胺能通过降低结肠黏膜上皮PAR2的表达而抑制内脏高敏感的形成与维持.
目的 觀察匹維溴胺對大鼠內髒高敏感形成及蛋白酶激活受體2(PAR2)錶達的影響.方法 採用慢性避水應激法(WAS)構建內髒高敏感大鼠模型.Wistar大鼠隨機分為假避水應激組、避水應激組和匹維溴胺治療組(30 mg·kg~(-1)·d~(-1)),每組12隻.觀察每組大鼠應激時的排便顆粒數,併以結直腸擴張時的內髒運動反射(VMR)幅度值為指標觀察內髒敏感性的變化.採用Western印跡和免疫熒光法檢測結腸黏膜上皮PAR2的錶達.結果 應激組大鼠排便顆粒數高于假避水應激組[(8.104±2.75)顆比(4.13±1.36)顆,P<0.001],匹維溴胺榦預能減少應激時的排便顆粒數[(5.87±1.78)顆,P<0.001].避水應激後大鼠對60 mmHg(1 mmHg=0.133 kPa)壓力擴張刺激的VMR幅度值(8.64±2.88)高于假避水應激組(3.805±1.04,P<0.001),匹維溴胺治療可降低模型大鼠的VMR幅度值(6.09±0.77,P=0.02).與假避水應激組相比,避水應激上調結腸PAR2錶達(相對灰度值分彆為0.32±0.04和0.70±0.06,纍積A值分彆為77.16±10.74及279.085±100.91,P<0.001),匹維溴胺治療抑製瞭PAR2的錶達上調(相對灰度值0.53±0.06,纍積A值180.59±25.75,P<0.05).結論 匹維溴胺能通過降低結腸黏膜上皮PAR2的錶達而抑製內髒高敏感的形成與維持.
목적 관찰필유추알대대서내장고민감형성급단백매격활수체2(PAR2)표체적영향.방법 채용만성피수응격법(WAS)구건내장고민감대서모형.Wistar대서수궤분위가피수응격조、피수응격조화필유추알치료조(30 mg·kg~(-1)·d~(-1)),매조12지.관찰매조대서응격시적배편과립수,병이결직장확장시적내장운동반사(VMR)폭도치위지표관찰내장민감성적변화.채용Western인적화면역형광법검측결장점막상피PAR2적표체.결과 응격조대서배편과립수고우가피수응격조[(8.104±2.75)과비(4.13±1.36)과,P<0.001],필유추알간예능감소응격시적배편과립수[(5.87±1.78)과,P<0.001].피수응격후대서대60 mmHg(1 mmHg=0.133 kPa)압력확장자격적VMR폭도치(8.64±2.88)고우가피수응격조(3.805±1.04,P<0.001),필유추알치료가강저모형대서적VMR폭도치(6.09±0.77,P=0.02).여가피수응격조상비,피수응격상조결장PAR2표체(상대회도치분별위0.32±0.04화0.70±0.06,루적A치분별위77.16±10.74급279.085±100.91,P<0.001),필유추알치료억제료PAR2적표체상조(상대회도치0.53±0.06,루적A치180.59±25.75,P<0.05).결론 필유추알능통과강저결장점막상피PAR2적표체이억제내장고민감적형성여유지.
Objective To investigate the effect of pinaverium bromide on the development of visceral hypersensitivity and the expression of proteinase-activated-receptor 2(PAR2)in rats with water avoidance stress (WAS).Methods The Wistar rats were randomly divided into 3 groups with 12 each.The control group was submitted to sham-WAS and the group A1 was submitted to WAS.The rats in group A2 were submitted to WAS and received pinaverium bromide treatment (30 mg·kg~(-1)·d~(-1)).The stool pellets were counted during WAS and sham-WAS.The magnitude of visceromotor response (VMR) during colorectal distention was used to measure the changes of visceral hypersensitivity.The PAR2 expression was detected by immunofluorescence staining and Western blotting.Results Increased stool pellets were found in group A1 compared to the control group (8.10±2.75 vs.4.13±1.36,P<0.001).Pinaverium bromide treatment decreased stool pellets during WAS (5.87±1.78,P<0.001).The magnitude of VMR (60 mmHg) was higher in group Al compared to the control group (8.64±2.88 vs.3.80±1.04,P<0.001).Pinaverium bromide
treatment could decrease the magnitude of VMR effectively after WAS (6.09±0.77,P=0.02).The expression of PAR2 in group Al was significantly higher than that in the control group (rAU:0.70±0.06 vs.0.32±0.04;M:279.08±100.91 vs.77.16±10.74,P<0.001).The increased PAR2 expression induced by WAS could be inhibited by pinaverium bromide (rAU:0.53±0.06,IA:180.59±25.75,P<0.05).Conclusions Pinaverium bromide may decrease the expression of PAR2 and inhibit the development of visceral hypersensitivity in WAS rats.
