CAJ | 학술논문

目的：为寻找恶性疟原虫基因工程多表位疫苗保护性评价的模型动物，利用小鼠约氏疟原虫(Plasmodium yoelii)及小鼠伯氏疟原虫(Plasmodium berghei)模型对本实验室构建的恶性疟原虫(Plasmodium falciparum)多阶段、多表位基因工程候选疫苗进行了体内保护性及免疫模式探索试验。结果在约氏疟原虫模型，先用该疫苗基因的大肠杆菌表达蛋白免疫，后用含同一基因的重组痘苗病毒加强免疫组，与野生型痘苗病毒免疫组及空白对照组相比，小鼠的死亡时间延长(P<0.01)。结果初步显示，约氏疟原虫鼠疟模型可用作该基因工程多表位恶性疟原虫候选疫苗的保护性评价。
목적：위심조악성학원충기인공정다표위역묘보호성평개적모형동물，이용소서약씨학원충(Plasmodium yoelii)급소서백씨학원충(Plasmodium berghei)모형대본실험실구건적악성학원충(Plasmodium falciparum)다계단、다표위기인공정후선역묘진행료체내보호성급면역모식탐색시험。결과재약씨학원충모형，선용해역묘기인적대장간균표체단백면역，후용함동일기인적중조두묘병독가강면역조，여야생형두묘병독면역조급공백대조조상비，소서적사망시간연장(P<0.01)。결과초보현시，약씨학원충서학모형가용작해기인공정다표위악성학원충후선역묘적보호성평개。
To find a suitable animal model for the vaccine assessment of Plasmodium falciparum,Plasmodium yoelii and Plasmodium berghei of mouse were used to evaluate the protection for a multi-epitope vaccine candidate of Plasmodium falciparum. Results showed that mice of the group firstly immunized with the protein expressed in E.coli,then boosted with the recombinant vaccinia virus from the same epitopes survived longer than that of groups immunized with the wild vaccinia virus or the blank control (P<0.01).This indicates that Plasmodium yoelii of mouse could be used as a protective model for the evaluation of multi-epitope vaccine candidate of Plasmodium falciparum.