中国寄生虫病防治杂志
中國寄生蟲病防治雜誌
중국기생충병방치잡지
CHINESE JOURNAL OF PARASITIC DISEASE CONTROL
2005年
2期
116-119
,共4页
许兵红%史明珠%艾予川%杨廷桐
許兵紅%史明珠%艾予川%楊廷桐
허병홍%사명주%애여천%양정동
蜂胶%阴道毛滴虫%抗滴虫
蜂膠%陰道毛滴蟲%抗滴蟲
봉효%음도모적충%항적충
Trichomonas vaginalis%propolis%antitrichomonas
目的观察蜂胶体外抗阴道毛滴虫的效果. 方法阴道毛滴虫体外无菌培养后,加入蜂胶溶液使其浓度为2.4 mg/ml,同时设置溶剂对照组和正常培养空白对照组,分别于0 h、6 h、12 h和24 h观察各组虫数、活率并计算活虫数. 结果培养0 h、6 h、12 h、24 h不同时间蜂胶组的虫数(万/ml)分别为:50.50±2.92、16.25±2.40、12.88±0.85、11.13±1.31;活虫数(万/ml):46.20±2.91、7.04±1.75、2.87±0.69、1.24±0.59;活率(%):91.50±3.11、43.00±6.83、22.25±5.32、11.50±5.74.空白对照组的虫数分别为:43.25±8.29、39.00±13.22、55.50±18.01、81.88±22.63;活虫数:37.68±7.56、36.77±12.28、50.09±17.19、79.61±22.09;活率:87.00±0.82、94.50±3.51、90.00±4.32、97.25±0.96.蜂胶组与溶剂对照组以及空白对照组比较差异有显著性. 结论蜂胶具有明显的体外抗阴道毛滴虫效果,培养液蜂胶浓度为2.40 mg/ml时,随培养时间延长,滴虫不断减少,活率不断降低.
目的觀察蜂膠體外抗陰道毛滴蟲的效果. 方法陰道毛滴蟲體外無菌培養後,加入蜂膠溶液使其濃度為2.4 mg/ml,同時設置溶劑對照組和正常培養空白對照組,分彆于0 h、6 h、12 h和24 h觀察各組蟲數、活率併計算活蟲數. 結果培養0 h、6 h、12 h、24 h不同時間蜂膠組的蟲數(萬/ml)分彆為:50.50±2.92、16.25±2.40、12.88±0.85、11.13±1.31;活蟲數(萬/ml):46.20±2.91、7.04±1.75、2.87±0.69、1.24±0.59;活率(%):91.50±3.11、43.00±6.83、22.25±5.32、11.50±5.74.空白對照組的蟲數分彆為:43.25±8.29、39.00±13.22、55.50±18.01、81.88±22.63;活蟲數:37.68±7.56、36.77±12.28、50.09±17.19、79.61±22.09;活率:87.00±0.82、94.50±3.51、90.00±4.32、97.25±0.96.蜂膠組與溶劑對照組以及空白對照組比較差異有顯著性. 結論蜂膠具有明顯的體外抗陰道毛滴蟲效果,培養液蜂膠濃度為2.40 mg/ml時,隨培養時間延長,滴蟲不斷減少,活率不斷降低.
목적관찰봉효체외항음도모적충적효과. 방법음도모적충체외무균배양후,가입봉효용액사기농도위2.4 mg/ml,동시설치용제대조조화정상배양공백대조조,분별우0 h、6 h、12 h화24 h관찰각조충수、활솔병계산활충수. 결과배양0 h、6 h、12 h、24 h불동시간봉효조적충수(만/ml)분별위:50.50±2.92、16.25±2.40、12.88±0.85、11.13±1.31;활충수(만/ml):46.20±2.91、7.04±1.75、2.87±0.69、1.24±0.59;활솔(%):91.50±3.11、43.00±6.83、22.25±5.32、11.50±5.74.공백대조조적충수분별위:43.25±8.29、39.00±13.22、55.50±18.01、81.88±22.63;활충수:37.68±7.56、36.77±12.28、50.09±17.19、79.61±22.09;활솔:87.00±0.82、94.50±3.51、90.00±4.32、97.25±0.96.봉효조여용제대조조이급공백대조조비교차이유현저성. 결론봉효구유명현적체외항음도모적충효과,배양액봉효농도위2.40 mg/ml시,수배양시간연장,적충불단감소,활솔불단강저.
Objective To investigate the antitrichomonas effect of propolis from Henan Province of China on Trichomonas vaginalis after being cultured for different hours in vitro. Methods The culture of T. vaginalis were divided into three groups: the propolis group, the solvent group and the control group. The concentration of propolis in the medium of propolis group was 2.4 mg/ml, equivalent amount of solvent was added to the solvent group, and the control group was normally cultured. The number of the parasite, the number of the survivals and the survival rate at 0, 6, 12 and 24 hours were observed and recorded respectively after being cultured. Results After being cultured for 6, 12 and 24 hours, the number of the survivals(104/ml) in the propolis group was: 7.04±1.75, 2.87±0.69, 1.24±0.59; the survival rate (%) was: 43.00±6.83, 22.25±5.32, 11.50±5.74. In the control group, the number of the survivals: 36.77±12.28, 50.09±17.19, 79.61±22.09; the survival rate: 94.50±3.51, 90.00±4.32, 97.25±0.96 respectively. Significant difference was found between the propolis group and the others. Conclusion Propolis collected from Henan Province possesses obvious antitrichomonas activity in vitro, the number of individuals would significantly decrease and the survival rate would strongly decline in the medium with propolis concentration at 2.40 mg/ml.
