CAJ | 학술논문

以栓皮栎未成熟合子胚为外植体,在添加0.25mg/L 2,4-D和0.5 mg/L 6-BA的MS培养基上6周可诱导产生2种类型的胚性愈伤组织,一种表面具光泽、白色;另一种表面光滑湿润具光泽,色泽淡黄或无色透明.组织切片表明,胚性愈伤组织的细胞体积小,细胞核大,细胞质浓,细胞排列紧密;非胚性愈伤组织细胞的体积大,细胞核小,细胞质稀薄.胚性细胞团培养在不含激素的培养基上可诱导产生体细胞胚.体细胞胚直接起源于胚性细胞团表皮或近表皮的单细胞,经历与合子胚相似的球形胚、心形胚、鱼雷胚和子叶胚发育阶段.所有发育时期的体细胞胚的胚轴、子叶均产生次生体胚,它们起源于细胞质较浓的表皮单细胞.
이전피력미성숙합자배위외식체,재첨가0.25mg/L 2,4-D화0.5 mg/L 6-BA적MS배양기상6주가유도산생2충류형적배성유상조직,일충표면구광택、백색;령일충표면광활습윤구광택,색택담황혹무색투명.조직절편표명,배성유상조직적세포체적소,세포핵대,세포질농,세포배렬긴밀;비배성유상조직세포적체적대,세포핵소,세포질희박.배성세포단배양재불함격소적배양기상가유도산생체세포배.체세포배직접기원우배성세포단표피혹근표피적단세포,경력여합자배상사적구형배、심형배、어뢰배화자협배발육계단.소유발육시기적체세포배적배축、자협균산생차생체배,타문기원우세포질교농적표피단세포.
Immature zygotic embryos of Quercus variabilis B1.were excised and cultured on MS basal medium containing 0.25 mg/L 2,4-dichlorophenoxyacetic acid and 0.5 mg/L 6-benzyl aminopurine.Callus was initiated from these embryos within 6 weeks.Two types of embryogenic calluses were formed:one was the white mucilaginous callus and the other was light yellow to translucent,glossy,mucilaginous.Histological examination showed that the non-embryogenic cells derived from zygotic embryos were large in size,with small nuclei,thin cytoplasm,and the embryogenic cells were small in size,with large nuclei,thick cytoplasm and denser arrangement.Somatic embryos were induced from embryogenic masses(EMS)cultured on a medium not containing plant growth regulators(PGRs).The histological origin of somatic embryos was single epidermal or subepidermal cells.Somatic embryos displayed a standard development pattern,from globular ones to heartlike and torpedo-shaped ones and finally to embryo with two cotyledons,which was similar to the development of zygotic embryo in vivo.Secondary proembryos were formed on the axis and cotyledon of existing embryos and originated from single,densely stained cells of the epidermis at all stages.