CAJ | 학술논문

1975年,D(O)bereiner实验发现与禾本科植物联合共生的固氮菌并提出根际联合固氮的概念.近年来,随着一些新的研究手段包括化学分析、遗传工程、分子生物学、免疫学等方法的运用,推进了联合固氮领域的研究深度.综述了近年来发现的联合固氮菌的种类;联合固氮体系的形成过程(趋化、结合和侵入);影响联合固氮的主要因素:自然条件、土著微生物的竞争、植物基因型差异和环境条件的变化、结合态氮(氨、亚硝酸盐、硝酸盐等)和氧.并从固氮联合作用测定方法、联合固氮菌的资源筛选、联合固氮工程菌的研究、联合固氮分子生态学研究方法4个方面,论述了联合固氮的研究现状.
1975년,D(O)bereiner실험발현여화본과식물연합공생적고담균병제출근제연합고담적개념.근년래,수착일사신적연구수단포괄화학분석、유전공정、분자생물학、면역학등방법적운용,추진료연합고담영역적연구심도.종술료근년래발현적연합고담균적충류;연합고담체계적형성과정(추화、결합화침입);영향연합고담적주요인소:자연조건、토저미생물적경쟁、식물기인형차이화배경조건적변화、결합태담(안、아초산염、초산염등)화양.병종고담연합작용측정방법、연합고담균적자원사선、연합고담공정균적연구、연합고담분자생태학연구방법4개방면,논술료연합고담적연구현상.
In 1975 D(O)bereiner experimentally found the associative symbiotic nitrogen-fixation (NF) bacteria associated with graminaceous plant and put forward the concept of rhizosphere associated NF (ANF). Recently, with the application of chemical analysis, hereditary engineer, molecular biology and immunology and other research means had pushed the depth of in the field of ANF studies. The category of ANF, the formation process of ANF (chemotaxis, integration, and invasion); main effect factors of ANF: natural conditions, competition among indigenous microbes, differences of plant genotypes in associative relationship and variance of environmental conditions, combined forms of nitrogen (ammonia, nitrite, nitrate etc.) and oxygen discovered recently were reviewed in this paper. Recent research situation of the application of ANF were also discussed in four aspects of research methods, detection methods of the ANF, screening of the ANF-bacterial resources, and the ANF engineering strains as well as molecular ecology.