植物病理学报
植物病理學報
식물병이학보
ACTA PHYTOPATHOLOGICA SINICA
2010年
1期
103-105
,共3页
张保元%孙漫红%张拥华%谢响明%李世东
張保元%孫漫紅%張擁華%謝響明%李世東
장보원%손만홍%장옹화%사향명%리세동
Clonostachys rosea%submerged spores%aerial spores%chlamydospores
The growth.and sporulation of Clonostachys rosea strain 67-1 in PD broth was observed and igured out.A large amount of submerged spores were obtained in shake flask with proprietary Czapek medium.Meanwhile,the colonies of strain 67-1 were cultured in PDA plate and aerial spores werg collected by elution.Resistances of submerged spores and aerial spores to high temperature,dry condition and UV treatment were determined.The results showed that the survival of the submerged spores kept in 60℃for 30 min was 76.7%.while the aerial spores were hardly germinated in the same condition.After two weeks'drying treatment,the spore activities were 89.2%and 29.3%,respectively.Similarly,the activity of submerged spores was 72.6%and that of aerial spores was 19.7%when exposing to UV for 1 min.It is illuminated that deep submerged fermentation is more efficient than solid culture for strain 67-1 to produce chlamydospores,which act as main component in biopesticide mass production.
