中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2012年
1期
31-34
,共4页
秦宏冉%田梅%高刚%阮健磊%刘建香
秦宏冉%田梅%高剛%阮健磊%劉建香
진굉염%전매%고강%원건뢰%류건향
高氡温泉%p53%miR-34a/b%let-7a%k-ras
高氡溫泉%p53%miR-34a/b%let-7a%k-ras
고동온천%p53%miR-34a/b%let-7a%k-ras
Hot springs with high radon%p53%miR-34a/b%let-7a%k-ras
目的 探讨四川省若尔盖县降扎乡异常高氡温泉对周围居民外周血中肺癌相关基因和miRNA表达的影响.方法 获取高氡温泉及对照地区居民外周血样,用实时RT-PCR检测肺癌相关基因( p53、k-ras)及血浆中相关miRNA(let-7a、miR-34a/b)的表达,用Western blot检测靶蛋白p53和k-ras的表达.结果 高氡温泉周围居民p53和k-ras基因mRNA表达分别是对照组的0.97和1.33倍,但差异无统计学意义(t=0.13、-1.12,P＞0.05)；靶蛋白p53和k-ras的表达与基因表达变化趋势一致,分别是对照组的0.7倍和1.23倍,(t=0.72、0.46,P＞0.05).高氡组let-7a与对照相比有下降趋势(t=1.63,P＞0.05).值得注意的是,与对照组相比,高氡组miR-34a和miR-34b明显高表达(t=-3.20、-3.32,P＜0.05).结论 通过检测p53和k-ras基因以及miRNA的变化,推测高氡温泉周围居民受到了辐射损伤.
目的 探討四川省若爾蓋縣降扎鄉異常高氡溫泉對週圍居民外週血中肺癌相關基因和miRNA錶達的影響.方法 穫取高氡溫泉及對照地區居民外週血樣,用實時RT-PCR檢測肺癌相關基因( p53、k-ras)及血漿中相關miRNA(let-7a、miR-34a/b)的錶達,用Western blot檢測靶蛋白p53和k-ras的錶達.結果 高氡溫泉週圍居民p53和k-ras基因mRNA錶達分彆是對照組的0.97和1.33倍,但差異無統計學意義(t=0.13、-1.12,P＞0.05)；靶蛋白p53和k-ras的錶達與基因錶達變化趨勢一緻,分彆是對照組的0.7倍和1.23倍,(t=0.72、0.46,P＞0.05).高氡組let-7a與對照相比有下降趨勢(t=1.63,P＞0.05).值得註意的是,與對照組相比,高氡組miR-34a和miR-34b明顯高錶達(t=-3.20、-3.32,P＜0.05).結論 通過檢測p53和k-ras基因以及miRNA的變化,推測高氡溫泉週圍居民受到瞭輻射損傷.
목적 탐토사천성약이개현강찰향이상고동온천대주위거민외주혈중폐암상관기인화miRNA표체적영향.방법 획취고동온천급대조지구거민외주혈양,용실시RT-PCR검측폐암상관기인( p53、k-ras)급혈장중상관miRNA(let-7a、miR-34a/b)적표체,용Western blot검측파단백p53화k-ras적표체.결과 고동온천주위거민p53화k-ras기인mRNA표체분별시대조조적0.97화1.33배,단차이무통계학의의(t=0.13、-1.12,P＞0.05)；파단백p53화k-ras적표체여기인표체변화추세일치,분별시대조조적0.7배화1.23배,(t=0.72、0.46,P＞0.05).고동조let-7a여대조상비유하강추세(t=1.63,P＞0.05).치득주의적시,여대조조상비,고동조miR-34a화miR-34b명현고표체(t=-3.20、-3.32,P＜0.05).결론 통과검측p53화k-ras기인이급miRNA적변화,추측고동온천주위거민수도료복사손상.
Objective To investigate the expressions of lung cancer related genes and miRNA in peripheral blood of the residents surrounding the extremely high radon hot springs in Ruoergai County,Sichuan Province. Methods Peripheral blood samples were collected from the local residents.Expressions of lung cancer related genes (p53,k-ras) and miRNA (let-7a,miR-34a/b) were detected by real-time PCR and the protein expressions of p53 and k-ras were detected by Western blot.Results The expressions of p53 and k-ras mRNA of the residents in high radon area were 0.97 and 1.33 times of the control respectively (t =0.13,-1.12,P ＞0.05),and the p53 and k-ras protein levels were 0.70 and 1.23 times of the control respectively (t =0.72,0.46,P ＞ 0.05).The let-7a of the residents in high radon area was lower (t =1.63,P ＞ 0.05 ) while the miR-34a and miR-34b were significantly higher than those of the controls (t =- 3.20,- 3.32,P ＜ 0.05).Conclusions Based on the expressions of p53 and k-ras gene and miRNA,it can be concluded that the residents surrounding the high radon hot springs received radiation damage.
