CAJ | 학술논문

目的探讨糖尿病患者谷氨酸脱羧酶抗体( GAD-Ab)的检出率及其对糖尿病分型的诊断价值.方法采用酶联免疫法检测98例糖尿病患者、20例正常对照者血谷氨酸脱羧酶的抗体水平,统计T1DM组、T2DM非肥胖组、T2DM肥胖组、正常对照组共4组研究对象的谷氨酸脱羧酶抗体的检出率,比较每种抗体各组间的阳性率,另外比较两种抗体同时阳性的比率.结果 ①T1DM组、T2DM非肥胖组、T2DM肥胖组GAD-Ab检出率分别为70.0％、6.3％和5.6％；ICA检出率分别为68％、8.8％、7.2％; GAD-Ab和ICA同时阳性的几率为68％.②糖尿病各组的检出率分别与正常组比较,P＜0.01,差异有显著性.③T1DM组的检出率分别与T2DM两组比较,均P＜0.05,差异有统计学意义.④T2DM两组间检出率比较,P＞0.05,差异无统计学意义.结论 GAD-Ab是1型糖尿病自然病程中β细胞功能损伤的一个指标,对糖尿病预测、分型、指导治疗、判断预后等具有一定的意义.
목적 탐토당뇨병환자곡안산탈최매항체( GAD-Ab)적검출솔급기대당뇨병분형적진단개치.방법 채용매련면역법검측98례당뇨병환자、20례정상대조자혈곡안산탈최매적항체수평,통계T1DM조、T2DM비비반조、T2DM비반조、정상대조조공4조연구대상적곡안산탈최매항체적검출솔,비교매충항체각조간적양성솔,령외비교량충항체동시양성적비솔.결과 ①T1DM조、T2DM비비반조、T2DM비반조GAD-Ab검출솔분별위70.0％、6.3％화5.6％；ICA검출솔분별위68％、8.8％、7.2％; GAD-Ab화ICA동시양성적궤솔위68％.②당뇨병각조적검출솔분별여정상조비교,P＜0.01,차이유현저성.③T1DM조적검출솔분별여T2DM량조비교,균P＜0.05,차이유통계학의의.④T2DM량조간검출솔비교,P＞0.05,차이무통계학의의.결론 GAD-Ab시1형당뇨병자연병정중β세포공능손상적일개지표,대당뇨병예측、분형、지도치료、판단예후등구유일정적의의.
Objective To investigate the detection rate of glutamic acid decarboxylase ( GAD )antibodies in patients with diabetes and the diagnostic value of the rate for typing diabetes.Methods Levels of GAD antibodies were detected in 98 patients with diabetes and 20 healthy controls by enzymelinked immunosorbent assay.The detection rate was documented in group with T1DM,non-obesity group with T2DM, obesity group with T2DM, and control group.The positive rate of GAD-Ab or ICA was compared among the four group and the GAD-Ab and ICA co-positive rate was also compared.Results The detection rate of GAD-Ab was 70.0％ in the group with T1DM,6.3％ in the non-obesity group with T2DM,5.6％ in the obesity group with T2DM; and that of ICA was 68％,8.8％,and 7.2％,respectively.The detection rate differed significantly between the groups with diabetes and the control group ( P＜ 0.01 )and between the group with T1DM and the two groups with T2DM ( P ＜ 0.05 ); while it did not differ significantly between the non-obesity group with T2DM and the obesity group with T2DM ( P ＞ 0.05 ).Conclusions Glutamic acid decarboxylase antibody is a marker of β-cell dysfunction in the natural course of type 1 diabetes mellitus.It is of significance in predicting,typing and treating diabetes mellitus,and estimating prognosis.