CAJ | 학술논문

目的研究肺复张与小潮气量机械通气联合应用对海水淹溺致急性肺损伤兔的氧合和呼吸力学的影响及安全性评价.方法 18只新西兰兔气管内缓慢灌入2 ml/kg海水,复制成海水淹溺致急性肺损伤兔模型,随后给予6 ml/kg小潮气量机械通气3 h.按是否给予肺复张分为对照组和肺复张组(RM组),每组9只.对照组小潮气量机械通气结束后即行肺高分辨率CT检查.RM组小潮气量通气结束后即给予肺复张,方法为:持续气道正压(CPAP),压力2.94 kPa,时间20 s,小潮气量机械通气期间观察记录2组氧合、呼吸力学、循环、血清炎性因子的变化.结果 RM组PaO2在各观察点明显改善,与对照组比较差异有统计学意义(P<0.05);能维持2 h,RM组在复张后15 min和30 min PaC02高于对照组,差异有统计学意义(P<0.05);平台压(Pplat)降低、静态顺应性(Crs,stat)增加,与对照组比较差异有统计学意义(P<0.05);RM组15 min平均动脉压下降(P<0.05),1 h后基本恢复基础水平;复张过程中,心率增加、血压下降,30～90 s恢复,在15 min时RM组心率较对照组低(P<0.05);2组血清肿瘤坏死因子α(TNF-α)、白介素-6(IL-6)差异无统计学意义(P>0.05);RM组肺高分辨率CT浸润影吸收好于对照组,无气压伤.结论肺复张与小潮气量通气联合应用能够明显改善兔氧合和肺顺应性,减轻肺损伤,是一种安全有效的治疗海水淹溺致急性肺损伤的机械通气手段.
목적 연구폐복장여소조기량궤계통기연합응용대해수엄닉치급성폐손상토적양합화호흡역학적영향급안전성평개.방법 18지신서란토기관내완만관입2 ml/kg해수,복제성해수엄닉치급성폐손상토모형,수후급여6 ml/kg소조기량궤계통기3 h.안시부급여폐복장분위대조조화폐복장조(RM조),매조9지.대조조소조기량궤계통기결속후즉행폐고분변솔CT검사.RM조소조기량통기결속후즉급여폐복장,방법위:지속기도정압(CPAP),압력2.94 kPa,시간20 s,소조기량궤계통기기간관찰기록2조양합、호흡역학、순배、혈청염성인자적변화.결과 RM조PaO2재각관찰점명현개선,여대조조비교차이유통계학의의(P<0.05);능유지2 h,RM조재복장후15 min화30 min PaC02고우대조조,차이유통계학의의(P<0.05);평태압(Pplat)강저、정태순응성(Crs,stat)증가,여대조조비교차이유통계학의의(P<0.05);RM조15 min평균동맥압하강(P<0.05),1 h후기본회복기출수평;복장과정중,심솔증가、혈압하강,30～90 s회복,재15 min시RM조심솔교대조조저(P<0.05);2조혈청종류배사인자α(TNF-α)、백개소-6(IL-6)차이무통계학의의(P>0.05);RM조폐고분변솔CT침윤영흡수호우대조조,무기압상.결론 폐복장여소조기량통기연합응용능구명현개선토양합화폐순응성,감경폐손상,시일충안전유효적치료해수엄닉치급성폐손상적궤계통기수단.
Objective To observe the therapeutic effect and safety of lung recruitment combined with lower tidal ventilation on acute lung injury induced by seawater drowning in rabbits. Methods Eighteen New Zealand rabbits were used for the experiment. Acute lung injury model was developed with intractracheal instillation of seawater (2 ml/kg). Then, the animals had been lower tidal ventilation (6 ml/kg) for three hours. The animals with lung recruitment maneuvers were designated as the experimental group (or the RM group), while those without lung recruitment were designated as the control group. For the RM group,continuous positive airway pressure (CPAP) was applied at a pressure of 2.94 kPa for 20 seconds. Then,arterial blood gas analysis, pulmodynamics, and hemodynamic status were made at various time points. Tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) extracted from serum were also measured following treatment. Pulmonary high - resolution chromatography (HRCT) was also made after three hours of ventilation.Results For the RM group, the partial pressure of arterial oxygen (PaO2) at various time points increased significantly after lung recruitment within two hours(P < 0.05). When a comparison was made between the two groups, PaCO2 for the RM group was higher than that of the control group following lung recruitment at 15 min and 30 min respectively with statistical significance (P < 0.05). Airway plateau pressure (Pplat) decreased markedly(P <0.05)and static compliance increased with statistical significance (P < 0.05). Mean arterial pressure for the RM group decreased in 15 min (P < 0. 05), but almost restored to basic level within 1 h.During recruitment, mean arterial pressure decreased and heart rate increased, but came to normal levels after 30- 90 s. Heart rate at 15 min for the RM group was lower than that of the control group (P < 0.05). However,for both groups, no significant differences could be noted in TN F-α and IL-6 (P > 0.05). Conclusions Lung recruitment combined with lower tidal ventilation could significantly improve gas exchange and lung compliance,and alleviate lung injury, which might be a safe and effective method for the treatment of animals with acute lung injury induced by seawater drowning.