中华航空航天医学杂志
中華航空航天醫學雜誌
중화항공항천의학잡지
CHINESE JOURNAL OF AEROSPACE MEDICINE
2008年
1期
35-38
,共4页
李天志%LIU Chang-ting%王德龙%汪德生%袁明%李志利
李天誌%LIU Chang-ting%王德龍%汪德生%袁明%李誌利
리천지%LIU Chang-ting%왕덕룡%왕덕생%원명%리지리
失重模拟%血液灌注%肺动脉%血管阻力%大鼠 Wistar
失重模擬%血液灌註%肺動脈%血管阻力%大鼠 Wistar
실중모의%혈액관주%폐동맥%혈관조력%대서 Wistar
Weightlessness simulation%Hemoperfusion%Pulmonary artery%Vascular resistance%Rats,Wistar
目的 研究模拟失重对离体大鼠肺血管调节功能的影响,旨在探讨模拟失重对肺循环的影响和立位耐力降低的发生机制. 方法采用尾部悬吊(TS)大鼠模拟失重生理效应.24只健康雄性Wistar大鼠随机分为对照组、TS 7 d和TS 14 d共3组.采用离体肺灌流技术,利用Power-lab生理系统记录恒流下大鼠肺循环灌注压的变化,并分别于苯肾上腺素(PE)、乙酰胆碱(Ach)和硝普钠(SNP)药物干预后,观察离体肺血管反应性,并计算出肺血管阻力. 结果与对照组相比,TS7 d和TS 14 d大鼠离体肺在恒流灌注下肺动脉压明显降低(F=3.58~20.86,P<0.05或P<0.01),离体大鼠肺动脉血管对PE的收缩反应明显下降,对Ach和SNP的舒张反应明显增强,同时肺血管阻力也相应降低(F=3.56~20.44,P<0.05或P<0.01). 结论尾吊大鼠离体肺血管灌注压降低,血管收缩反应性降低、舒张反应性增强,肺血管阻力减弱,尾吊模拟失重大鼠肺循环血管调节功能减弱,对失重后立位耐力不良有一定的影响.
目的 研究模擬失重對離體大鼠肺血管調節功能的影響,旨在探討模擬失重對肺循環的影響和立位耐力降低的髮生機製. 方法採用尾部懸弔(TS)大鼠模擬失重生理效應.24隻健康雄性Wistar大鼠隨機分為對照組、TS 7 d和TS 14 d共3組.採用離體肺灌流技術,利用Power-lab生理繫統記錄恆流下大鼠肺循環灌註壓的變化,併分彆于苯腎上腺素(PE)、乙酰膽堿(Ach)和硝普鈉(SNP)藥物榦預後,觀察離體肺血管反應性,併計算齣肺血管阻力. 結果與對照組相比,TS7 d和TS 14 d大鼠離體肺在恆流灌註下肺動脈壓明顯降低(F=3.58~20.86,P<0.05或P<0.01),離體大鼠肺動脈血管對PE的收縮反應明顯下降,對Ach和SNP的舒張反應明顯增彊,同時肺血管阻力也相應降低(F=3.56~20.44,P<0.05或P<0.01). 結論尾弔大鼠離體肺血管灌註壓降低,血管收縮反應性降低、舒張反應性增彊,肺血管阻力減弱,尾弔模擬失重大鼠肺循環血管調節功能減弱,對失重後立位耐力不良有一定的影響.
목적 연구모의실중대리체대서폐혈관조절공능적영향,지재탐토모의실중대폐순배적영향화립위내력강저적발생궤제. 방법채용미부현조(TS)대서모의실중생리효응.24지건강웅성Wistar대서수궤분위대조조、TS 7 d화TS 14 d공3조.채용리체폐관류기술,이용Power-lab생리계통기록항류하대서폐순배관주압적변화,병분별우분신상선소(PE)、을선담감(Ach)화초보납(SNP)약물간예후,관찰리체폐혈관반응성,병계산출폐혈관조력. 결과여대조조상비,TS7 d화TS 14 d대서리체폐재항류관주하폐동맥압명현강저(F=3.58~20.86,P<0.05혹P<0.01),리체대서폐동맥혈관대PE적수축반응명현하강,대Ach화SNP적서장반응명현증강,동시폐혈관조력야상응강저(F=3.56~20.44,P<0.05혹P<0.01). 결론미조대서리체폐혈관관주압강저,혈관수축반응성강저、서장반응성증강,폐혈관조력감약,미조모의실중대서폐순배혈관조절공능감약,대실중후립위내력불량유일정적영향.
Objective To study the effects of simulated microgravity on regulative function of isolated perfused pulmonary arteries in rats, in order to investigate the mechanism of the changes of pulmonary circulation and poor orthostatic tolerance under simulated microgravity environment.Methods Tail-suspension (TS) rats were used as the animal model to simulate the physiological effects of microgravity. Twenty-four healthy male Wistar rats were randomly divided into 3 groups:control group (CON), TS for 7 d(TS 7 d) and TS14d. The changes of the regulative function of pulmonary arteries to several vasoactive agents were measured by using the perfusion technique of isolated lung and the data were recorded by Powerlab system. Results Compared with the control group, the perfused pressure of the isolated pulmonary arteries decreased significantly in TS 7 d and TS 14 d groups (F=3.58~20.86, P<0.05 or P<0.01), the contractile responses of pulmonary arteries to phenylephrine(PE) declined in TS 14 d, while the vasodilator responses to acetylcholine(Ach) and sodium nitroprusside (SNP) were markedly enhanced. Moreover, pulmonary vascular resistance(PVR) decreased significantly in TS 7d and TS 14 d rats (F= 3.56~20.44, P<0.05 or P<0.01). Conclusion The isolated pulmonary arteries in TS rats under microgravity showsdecreased perfused pressure and contractile response but increased vasodilator responsiveness, while isolated perfusion PVR attenuated. The impaired regulative functions of pulmonary arteries may influence orthostatic intolerance.