CAJ | 학술논문

目的探讨雷公藤内酯醇(TL)对胰腺癌细胞生长及肿瘤新生血管生成的抑制作用.方法采用CCK-8试剂盒检测TL对胰腺癌SW1990细胞的生长抑制作用;采用TUNEL法检测TL对细胞凋亡的诱导作用;观察尾静脉注射TL对裸鼠移植瘤生长抑制作用和对瘤组织VEGF表达、微血管密度(MVD)的影响.结果 TL呈浓度和时间依赖性抑制SW1990细胞增殖,160 mg/ml的TL干预SW1990细胞24 h,细胞抑制率达50.6%,细胞凋亡率从对照组的9.6%升高到45.1%(P<0.01);TL0.5 mg/kg体重瘤内注射对移植瘤的抑瘤率达89.9%,瘤组织VEGF表达减少,MVD从36.25±8.64减少到9.87±3.34(P<0.01).结论 TL可显著抑制胰腺癌细胞增殖并诱导细胞凋亡;通过抑制肿瘤新生血管生成可抑制裸鼠SW1990细胞移植瘤的生长.
목적 탐토뢰공등내지순(TL)대이선암세포생장급종류신생혈관생성적억제작용.방법 채용CCK-8시제합검측TL대이선암SW1990세포적생장억제작용;채용TUNEL법검측TL대세포조망적유도작용;관찰미정맥주사TL대라서이식류생장억제작용화대류조직VEGF표체、미혈관밀도(MVD)적영향.결과 TL정농도화시간의뢰성억제SW1990세포증식,160 mg/ml적TL간예SW1990세포24 h,세포억제솔체50.6%,세포조망솔종대조조적9.6%승고도45.1%(P<0.01);TL0.5 mg/kg체중류내주사대이식류적억류솔체89.9%,류조직VEGF표체감소,MVD종36.25±8.64감소도9.87±3.34(P<0.01).결론 TL가현저억제이선암세포증식병유도세포조망;통과억제종류신생혈관생성가억제라서SW1990세포이식류적생장.
Objective To investigate the suppression effects of Tripotolide (TL) on the pancreatic cancer xenograft models and angiogenesis. Methods The growth suppression effect of TL on SW1990 was determined using cell count kit (CCK-8), apoptotic cells induced by TL were examined by morphology and terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay. The inhibitory effects of TL on the growth of tumor xenografts and tumor microvascular density (MVD) were investigated. Results TL inhibited the growth and proliferation of SW1990 cells in a concentration-dependent and time-dependent manner. The inhibition ratios of cells treated at 160 mg/ml TL for 24 h was 50. 6%, the apoptotic rate increased from 9.6% in the control group to 45.1% (P <0.01 ). The inhibition rate of cancer xenograft growth was 89.9% when TL was intratumorally injected at the dose of 0.5 mg/kg. The expression of VEGF in tumor tissue decreased while MVD also decreased from 36.25±8.64 to 9.87±3.34 (P <0.01 ). Conclusions TL induced prominent growth inhibition and apoptosis in human pancreatic cancer cell lines. TL.can attenuate the growth of pancreatic caner xenografts through its effect on antiangiogenesis.