中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2008年
11期
1850-1852
,共3页
单纯疱疹病毒属%Ki-67抗原%宫颈肿瘤%癌前状态
單純皰疹病毒屬%Ki-67抗原%宮頸腫瘤%癌前狀態
단순포진병독속%Ki-67항원%궁경종류%암전상태
Simplexvirus%Ki-67 antigen%Cervix neoplasms%Precancerous conditions
目的 研究子宫颈癌及癌前病变中单纯疱疹病毒2型(HSV-2)感染和增殖细胞核抗原Ki-67的表达情况.方法 采用PCR技术及免疫组织化学检测84例子宫颈癌组织(鳞状细胞癌67例、鳞腺癌3例、腺癌14例)、35例宫颈上皮内瘤变(CN)组织、13例正常宫颈组织的HSV-2、Ki-67的表达情况.结果 (1)HSV-2阳性率为40.2%(53/132),其中HSV-2阳性率在子宫颈癌组织中为48.8%(41/84),在CN组织中为28.5%(10/35),在正常宫颈黏膜上皮中为15.4%(2/13).癌组织、癌前病变HSV-2 DNA表达明显高于正常宫颈组织(P<0.01).(2)Ki-67的阳性细胞在正常子宫颈、CN和子宫颈癌组织中表达逐级增加,显示Ki-67表达程度与组织学类型有关.HSV-2和Ki-67在CN和癌组织中的表达呈显著相关(P<0.01).结论 宫颈癌及癌前病变组织中存在HSV-2感染,HSV-2感染和Ki-67表达率密切相关,两者的共同作用可能是宫颈癌发生、发展的重要因素.
目的 研究子宮頸癌及癌前病變中單純皰疹病毒2型(HSV-2)感染和增殖細胞覈抗原Ki-67的錶達情況.方法 採用PCR技術及免疫組織化學檢測84例子宮頸癌組織(鱗狀細胞癌67例、鱗腺癌3例、腺癌14例)、35例宮頸上皮內瘤變(CN)組織、13例正常宮頸組織的HSV-2、Ki-67的錶達情況.結果 (1)HSV-2暘性率為40.2%(53/132),其中HSV-2暘性率在子宮頸癌組織中為48.8%(41/84),在CN組織中為28.5%(10/35),在正常宮頸黏膜上皮中為15.4%(2/13).癌組織、癌前病變HSV-2 DNA錶達明顯高于正常宮頸組織(P<0.01).(2)Ki-67的暘性細胞在正常子宮頸、CN和子宮頸癌組織中錶達逐級增加,顯示Ki-67錶達程度與組織學類型有關.HSV-2和Ki-67在CN和癌組織中的錶達呈顯著相關(P<0.01).結論 宮頸癌及癌前病變組織中存在HSV-2感染,HSV-2感染和Ki-67錶達率密切相關,兩者的共同作用可能是宮頸癌髮生、髮展的重要因素.
목적 연구자궁경암급암전병변중단순포진병독2형(HSV-2)감염화증식세포핵항원Ki-67적표체정황.방법 채용PCR기술급면역조직화학검측84례자궁경암조직(린상세포암67례、린선암3례、선암14례)、35례궁경상피내류변(CN)조직、13례정상궁경조직적HSV-2、Ki-67적표체정황.결과 (1)HSV-2양성솔위40.2%(53/132),기중HSV-2양성솔재자궁경암조직중위48.8%(41/84),재CN조직중위28.5%(10/35),재정상궁경점막상피중위15.4%(2/13).암조직、암전병변HSV-2 DNA표체명현고우정상궁경조직(P<0.01).(2)Ki-67적양성세포재정상자궁경、CN화자궁경암조직중표체축급증가,현시Ki-67표체정도여조직학류형유관.HSV-2화Ki-67재CN화암조직중적표체정현저상관(P<0.01).결론 궁경암급암전병변조직중존재HSV-2감염,HSV-2감염화Ki-67표체솔밀절상관,량자적공동작용가능시궁경암발생、발전적중요인소.
Objective To explore the herpes virus Ⅱ(HSV-2) infection and Ki-67 expression for the cervical carcinoma and cervical intraepithelial neoplasia(CN).Methods The polymerase chain reaction(PCR) and immunohistochemistry were applied to detect the HSV-2 and Ki-67 expressions in cancerous tissues of uterine cervix in 84 cases (67 scaly cell carcinoma,3 scaly adenocarcinoma,and 14 adenocarcinoma cases),CN in 35 cases,and normal cervical tissues in 13 cases.Results (1)The positive rote of HSV-2 is 40.2% (53/132),and the rate in cervical cancerous tissues,CN and normal cervical tissues were 48.8% (41/48) ,28.5% (10/35) and 15.4% (2/13) respectively.HSV-2 DNA expressions in cancerous tissue and CN were much higher than that of normal cervical tissues (P<0.01).(2)The Ki-67 positive cells were progressive increasing in the normal cervical tissues,CN and cervical cancerous tissues,thus showing that there were relations between the Ki-67 expression degree and tissue types.The HSV-2 and Ki-67 expressions were remarkably connected(P < 0.01).Conclusion There are HSV-2 infection ,close related between HSV-2 infection and Ki-67 expression in the tissues of cervical carcinoma and CN.And the cooperation of them may play an important role in the development of cervical carcinoma.
