中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2009年
3期
285-289
,共5页
结肠肿瘤%基因%TROP2%预后
結腸腫瘤%基因%TROP2%預後
결장종류%기인%TROP2%예후
Colonic neoplasms%Gene,TROP2%Prognosis
目的 探讨TROP2基因在左半结肠和右半结肠癌组织中的表达及其临床意义.方法 选择2001年6月至2005年4月间在北京大学临床肿瘤学院接受根治性手术切除的Ⅱ、Ⅲ期结肠癌患者80例,其中右半结肠癌(RSCC)和左半结肠癌(LSCC)各40例.应用实时定量RT-PCR的方法检测TROP2 mRNA在癌组织和正常组织中的表达,并分析其与患者临床病理变量的关系及其对预后的影响.结果 TROP2基因mRNA在左、右半结肠癌组织中的表达均显著高于自身正常结肠黏膜(P<0.01);然而,TROP2在LSCC中的表达明显高于RSCC(P=0.009).LSCC中的TROP2高表达病例明显多于RSCC(67.5%:32.5%,P=0.002);高表达组患者的癌相关病死率是低表达组的4倍(40%:10%,P=0.002).Kaplan-Meier法分层分析显示,LSCC患者TROP2高表达者的中位生存时间明显短于低表达者(45.9个月:63.1个月,P=0.032);而RSCC患者TROP2表达对生存时间没有影响(P=0.235).多因素分析发现,肿瘤浸润深度和脉管癌栓是RSCC的独立预后因子;肿瘤浸润深度、淋巴结转移和脉管癌栓是LSCC的独立预后因子,TROP2高表达具有临界显著性(RR:6.244;95%CI:0.755-51.636;P=0.089).结论 TROP2与不良预后变量密切相关.TBOP2基因在LSCC中的表达明显高于RSCC:TROP2高表达是LSCC患者潜在的独立预后因子;LSCC和RSCC可能是存在显著分子差异的两个不同的疾病.
目的 探討TROP2基因在左半結腸和右半結腸癌組織中的錶達及其臨床意義.方法 選擇2001年6月至2005年4月間在北京大學臨床腫瘤學院接受根治性手術切除的Ⅱ、Ⅲ期結腸癌患者80例,其中右半結腸癌(RSCC)和左半結腸癌(LSCC)各40例.應用實時定量RT-PCR的方法檢測TROP2 mRNA在癌組織和正常組織中的錶達,併分析其與患者臨床病理變量的關繫及其對預後的影響.結果 TROP2基因mRNA在左、右半結腸癌組織中的錶達均顯著高于自身正常結腸黏膜(P<0.01);然而,TROP2在LSCC中的錶達明顯高于RSCC(P=0.009).LSCC中的TROP2高錶達病例明顯多于RSCC(67.5%:32.5%,P=0.002);高錶達組患者的癌相關病死率是低錶達組的4倍(40%:10%,P=0.002).Kaplan-Meier法分層分析顯示,LSCC患者TROP2高錶達者的中位生存時間明顯短于低錶達者(45.9箇月:63.1箇月,P=0.032);而RSCC患者TROP2錶達對生存時間沒有影響(P=0.235).多因素分析髮現,腫瘤浸潤深度和脈管癌栓是RSCC的獨立預後因子;腫瘤浸潤深度、淋巴結轉移和脈管癌栓是LSCC的獨立預後因子,TROP2高錶達具有臨界顯著性(RR:6.244;95%CI:0.755-51.636;P=0.089).結論 TROP2與不良預後變量密切相關.TBOP2基因在LSCC中的錶達明顯高于RSCC:TROP2高錶達是LSCC患者潛在的獨立預後因子;LSCC和RSCC可能是存在顯著分子差異的兩箇不同的疾病.
목적 탐토TROP2기인재좌반결장화우반결장암조직중적표체급기림상의의.방법 선택2001년6월지2005년4월간재북경대학림상종류학원접수근치성수술절제적Ⅱ、Ⅲ기결장암환자80례,기중우반결장암(RSCC)화좌반결장암(LSCC)각40례.응용실시정량RT-PCR적방법검측TROP2 mRNA재암조직화정상조직중적표체,병분석기여환자림상병리변량적관계급기대예후적영향.결과 TROP2기인mRNA재좌、우반결장암조직중적표체균현저고우자신정상결장점막(P<0.01);연이,TROP2재LSCC중적표체명현고우RSCC(P=0.009).LSCC중적TROP2고표체병례명현다우RSCC(67.5%:32.5%,P=0.002);고표체조환자적암상관병사솔시저표체조적4배(40%:10%,P=0.002).Kaplan-Meier법분층분석현시,LSCC환자TROP2고표체자적중위생존시간명현단우저표체자(45.9개월:63.1개월,P=0.032);이RSCC환자TROP2표체대생존시간몰유영향(P=0.235).다인소분석발현,종류침윤심도화맥관암전시RSCC적독립예후인자;종류침윤심도、림파결전이화맥관암전시LSCC적독립예후인자,TROP2고표체구유림계현저성(RR:6.244;95%CI:0.755-51.636;P=0.089).결론 TROP2여불량예후변량밀절상관.TBOP2기인재LSCC중적표체명현고우RSCC:TROP2고표체시LSCC환자잠재적독립예후인자;LSCC화RSCC가능시존재현저분자차이적량개불동적질병.
Objective To investigate the expression of TROP2 in the left-sided and right-sided colon cancer and its clinical significance. Methods A total of eighty patients, who received radical resection of colon cancer between June 2001 and April 2005 and were staged as Ⅱ and Ⅲ, were identified, including forty with left-sided colon cancer(LSCC)and forty with right-sided colon cancer (RSCC). The expression of TROP2 was detected by real-time quantitative RT-PCR in paired cancer and normal tissue. Subsequently, the relationship between TROP2 expessian and clinicopathoiogical variables as well as the effect on the patients' prognosis were analyzed. Results The expression of TROP2 mRNA in the cancer tissue was significantly higher than that in normal tissue (P<0.01, paired Wilcoxon test). However, its expression in LSCC was markedly higher than that in RSCC with significant difference (P=0.009, Mann-Whitney U test). The patients with TROP2 high expression were found more frequently in LSCC than in RSCC (67.5% vs 32.5%, P=0.002, χ2 test). Cancer-related mortality of the patients with TROP2 high expression was four times as high as low expression (40% vs 10%, P=0.002, χ2 test). From the stratified survival analysis through Kaplan-Meier curve, the TBOP2 high expression group had a significantly poorer median survival time than the low expression group for the patients with LSCC (45.9:63.1 months, P=0.032, log-rank test). By contrast, for the patients with RSCC, TROP2 expression had no marked effect on the survival time (P=0.235, log-rank test). In multivariable analysis, for the cohort of the present study, serosal invasion and lymphatic/vascular invasion were the independent prognostic factors of RSCC. Serosal invasion, lymph node metastasis and lymphatic/vascular invasion were the independent prognostic factors of LSCC. TROP2 high expression showed marginal significance (RR:6.244, 95% CI:0.755-51.636, P=0.089). Conclusion (1)TROP2 is a differentially expressed gene between RSCC and LSCC. (2)TROP2 high expression is closely related to the factors indicating poor prognosis. (3)TROP2 has distinct clinical significance to the patients with different tumor sites. TROP2 high expression is potentially an independent prognostic factor of LSCC. (4)LSCC and RSCC seem to be two distinct diseases with significant molecular heterogeneity.
