中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
5期
601-602
,共2页
邵珊%白波%李荣山%于为民%黄轶嵘
邵珊%白波%李榮山%于為民%黃軼嶸
소산%백파%리영산%우위민%황질영
全反式维甲酸%足细胞%高糖
全反式維甲痠%足細胞%高糖
전반식유갑산%족세포%고당
All trans-retinoic acid%Podocyte%High glucose
目的 探讨高糖培养的人肾小球足细胞synaptopodin蛋白表达的变化以及全反式维甲酸对其表达的干预效应.方法 以体外培养的条件性永生人肾小球足细胞为研究对象,平均分为正常糖(NG)组、高糖(HG)组、HG+5tmol/L全反式维甲酸干预组、HG+ 15 μmol/L全反式维甲酸干预组.蛋白质印迹法检测HG组足细胞培养0、3、6、9d时和其他各组足细胞培养9 dsynaptopodin蛋白的表达.结果 HG组足细胞培养3、6、9d时synaptopodin蛋白表达灰度值与培养0d时比较明显降低[(1.15±0.04)％、(0.78±0.04)％、(0.41±0.05)％比(1.49±0.07)％,P＜0.05].培养9d后,与NG组比较,HG组、HG+5μmol/L维甲酸干预组、HG+ 15 μmol/L维甲酸干预组足细胞synaptopodin蛋白表达灰度值均明显下降[(0.41±0.05)％、(0.86±0.04)％、(0.90±0.07)％比(1.47 ±0.08)％,均P＜0.05].结论 高糖可以使体外培养的足细胞synaptopodin蛋白表达下降,全反式维甲酸具有潜在的保护足细胞和防治糖尿病肾病的作用.
目的 探討高糖培養的人腎小毬足細胞synaptopodin蛋白錶達的變化以及全反式維甲痠對其錶達的榦預效應.方法 以體外培養的條件性永生人腎小毬足細胞為研究對象,平均分為正常糖(NG)組、高糖(HG)組、HG+5tmol/L全反式維甲痠榦預組、HG+ 15 μmol/L全反式維甲痠榦預組.蛋白質印跡法檢測HG組足細胞培養0、3、6、9d時和其他各組足細胞培養9 dsynaptopodin蛋白的錶達.結果 HG組足細胞培養3、6、9d時synaptopodin蛋白錶達灰度值與培養0d時比較明顯降低[(1.15±0.04)％、(0.78±0.04)％、(0.41±0.05)％比(1.49±0.07)％,P＜0.05].培養9d後,與NG組比較,HG組、HG+5μmol/L維甲痠榦預組、HG+ 15 μmol/L維甲痠榦預組足細胞synaptopodin蛋白錶達灰度值均明顯下降[(0.41±0.05)％、(0.86±0.04)％、(0.90±0.07)％比(1.47 ±0.08)％,均P＜0.05].結論 高糖可以使體外培養的足細胞synaptopodin蛋白錶達下降,全反式維甲痠具有潛在的保護足細胞和防治糖尿病腎病的作用.
목적 탐토고당배양적인신소구족세포synaptopodin단백표체적변화이급전반식유갑산대기표체적간예효응.방법 이체외배양적조건성영생인신소구족세포위연구대상,평균분위정상당(NG)조、고당(HG)조、HG+5tmol/L전반식유갑산간예조、HG+ 15 μmol/L전반식유갑산간예조.단백질인적법검측HG조족세포배양0、3、6、9d시화기타각조족세포배양9 dsynaptopodin단백적표체.결과 HG조족세포배양3、6、9d시synaptopodin단백표체회도치여배양0d시비교명현강저[(1.15±0.04)％、(0.78±0.04)％、(0.41±0.05)％비(1.49±0.07)％,P＜0.05].배양9d후,여NG조비교,HG조、HG+5μmol/L유갑산간예조、HG+ 15 μmol/L유갑산간예조족세포synaptopodin단백표체회도치균명현하강[(0.41±0.05)％、(0.86±0.04)％、(0.90±0.07)％비(1.47 ±0.08)％,균P＜0.05].결론 고당가이사체외배양적족세포synaptopodin단백표체하강,전반식유갑산구유잠재적보호족세포화방치당뇨병신병적작용.
Objective To investigate the effect of all trans-retinoic acid on the expression of synaptopodin in human podocytes induced by high glucose.Methods Conditionally immortalized human podocytes were exposed to nomal glucose group (NG) and high glucose group (HG).The expression of synaptopodin in podocytes was detected by Western blot.Results The expression of synaptopodin decreased significantly in time-dependent manner after 3,6 and 9 days of high glucose (HG) stimulation [ ( 1.15 ± 0.04) ％,(0.78 ± 0.04) ％,(0.41 ± 0.05 ) ％ vs ( 1.49 ± 0.07) ％,P ＜ 0.05 ].Compared to HG group,5 μmol/L and 15 μmol/L all-trans retinoic acid (ATRA) increased synaptopodin expressionat 9th day dramaticaly [ (0.41 ± 0.05 ) ％,(0.86 ± 0.04) ％,(0.90 ± 0.07 ) ％ vs ( 1.47 ± 0.08) ％,P ＜ 0.05 ].Conclusions High concentration of glucose decreases the expression of synaptopodin in podocyte.All trans retinoic acid(ATRA)may exert protective role on diabetic nephropathy by increasing synaptopodin expression which is downregulated by HG.
