CAJ | 학술논문

目的参照文献建立一种快速、灵敏、准确的生殖支原体(Mg)的检测方法,并应用该方法调查生殖支原体在广西贺州暗娼中的流行情况.方法参考文献中以生殖支原体Pa基因为靶基因设计的引物和Taqman MGB探针进行实时定量PCR,以生殖支原体标准菌株G37制备标准品,对广西贺州暗娼人群的宫颈拭子标本进行生殖支原体的检测.结果建立的Taqman MGB实时定量PCR方法检测的线性范围好(1×10～106拷贝/μl),R2=0.993,重复性好,(批内变异系数=0.7％,批间变异系数=1.09％),敏感性高,检测限(LOD)为10拷贝/μl,定量限(LOQ)为50拷贝/μl.采用该方法检测404份拭子标本,生殖支原体的检出率为12.1％.结论针对生殖支原体Pa基因的Taqman MGB实时PCR可以快速、灵敏地对生殖支原体进行定性及定量的检测.
목적 삼조문헌건립일충쾌속、령민、준학적생식지원체(Mg)적검측방법,병응용해방법조사생식지원체재엄서하주암창중적류행정황.방법 삼고문헌중이생식지원체Pa기인위파기인설계적인물화Taqman MGB탐침진행실시정량PCR,이생식지원체표준균주G37제비표준품,대엄서하주암창인군적궁경식자표본진행생식지원체적검측.결과 건립적Taqman MGB실시정량PCR방법검측적선성범위호(1×10～106고패/μl),R2=0.993,중복성호,(비내변이계수=0.7％,비간변이계수=1.09％),민감성고,검측한(LOD)위10고패/μl,정량한(LOQ)위50고패/μl.채용해방법검측404빈식자표본,생식지원체적검출솔위12.1％.결론 침대생식지원체Pa기인적Taqman MGB실시PCR가이쾌속、령민지대생식지원체진행정성급정량적검측.
Objective To establish a rapid,sensitive and accurate method to detect Mycoplasma genitalium,and to evaluate the prevalence of M.genitalium among unlicensed prostitutes from Hezhou city in Guangxi Zhuang Autonomous Region.Methods A pair of primers and Taqman MGB probe were designed and synthesized for the Pa gene of M.genitalium.Standard samples were prepared with the M.genitalium type strain G37.The established Taqman MGB real time fluorescence-based PCR assay was used to detect M.genitalium in the standard samples and cervical swab specimens collected from unlicensed prostitutes in Hezhou city of Guangxi Zhuang Autonomous Region.Results The established Taqman MGB real time PCR exhibited a wide linear range ( 1 × 10 copies/μl to 1 × 106 copies/μl,R2 =0.993),good repeatability (intra-assay variation;0.7％,inter-assay variation:1.09％) and hign sensitivity with the limit of detection being 10 copies/μl and limit of quantification being 50 copies/μl.As the assay showed,12.1％ of the 404 cervical swab samples were positive for M.genitalium.Conculsion The Taqman MGB real time fluorescence-based PCR is a rapid and sensitive method for the quantitative and qualitative detection of M.genitalium.