中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2009年
1期
38-40
,共3页
肉毒毒素类%腮腺%流涎
肉毒毒素類%腮腺%流涎
육독독소류%시선%류연
Botulinum toxins%Parotid gland%Sialorrhea
目的 探讨大鼠腮腺注射A型肉毒素(botulinum toxin type A,BTX-A)后组织学和免疫变化的机制.方法 18只雌性Wistar大鼠分为A、B组.A组动物(n=6)右侧腮腺内注射0.1 ml的生理盐水;B组动物(n=12)右侧腮腺注射BTX-A(2.5 U/0.1 ml).两组动物分别在注射后第7、12、35天处死并行组织学及免疫组化观察.结果 A组、B组注射后第7天部分腺泡细胞轻度萎缩,血管活性肠肽免疫反应(vasoactive intestinal polypeptide immunoreaction,VIP-IR)阳性纤维密度显著减低(n=4,P＜0.05);12 d萎缩的腺泡细胞和腺管明显增多,VIP-IR阳性纤维密度减低更明显(n=4,P＜0.001);35 d腺细胞和VIP-IR纤维密度与生理盐水组基本相同.结论 局部注射BTX-A可以导致大鼠腮腺腺泡细胞和腺管短时性萎缩,VIP-IR表达减少,提示BTX-A通过抑制调控腮腺分泌的血管活性肠肽(vasoactive intestinal polypeptide,VIP)释放导致腺组织非损伤性萎缩,而产生腺体分泌减少.
目的 探討大鼠腮腺註射A型肉毒素(botulinum toxin type A,BTX-A)後組織學和免疫變化的機製.方法 18隻雌性Wistar大鼠分為A、B組.A組動物(n=6)右側腮腺內註射0.1 ml的生理鹽水;B組動物(n=12)右側腮腺註射BTX-A(2.5 U/0.1 ml).兩組動物分彆在註射後第7、12、35天處死併行組織學及免疫組化觀察.結果 A組、B組註射後第7天部分腺泡細胞輕度萎縮,血管活性腸肽免疫反應(vasoactive intestinal polypeptide immunoreaction,VIP-IR)暘性纖維密度顯著減低(n=4,P＜0.05);12 d萎縮的腺泡細胞和腺管明顯增多,VIP-IR暘性纖維密度減低更明顯(n=4,P＜0.001);35 d腺細胞和VIP-IR纖維密度與生理鹽水組基本相同.結論 跼部註射BTX-A可以導緻大鼠腮腺腺泡細胞和腺管短時性萎縮,VIP-IR錶達減少,提示BTX-A通過抑製調控腮腺分泌的血管活性腸肽(vasoactive intestinal polypeptide,VIP)釋放導緻腺組織非損傷性萎縮,而產生腺體分泌減少.
목적 탐토대서시선주사A형육독소(botulinum toxin type A,BTX-A)후조직학화면역변화적궤제.방법 18지자성Wistar대서분위A、B조.A조동물(n=6)우측시선내주사0.1 ml적생리염수;B조동물(n=12)우측시선주사BTX-A(2.5 U/0.1 ml).량조동물분별재주사후제7、12、35천처사병행조직학급면역조화관찰.결과 A조、B조주사후제7천부분선포세포경도위축,혈관활성장태면역반응(vasoactive intestinal polypeptide immunoreaction,VIP-IR)양성섬유밀도현저감저(n=4,P＜0.05);12 d위축적선포세포화선관명현증다,VIP-IR양성섬유밀도감저경명현(n=4,P＜0.001);35 d선세포화VIP-IR섬유밀도여생리염수조기본상동.결론 국부주사BTX-A가이도치대서시선선포세포화선관단시성위축,VIP-IR표체감소,제시BTX-A통과억제조공시선분비적혈관활성장태(vasoactive intestinal polypeptide,VIP)석방도치선조직비손상성위축,이산생선체분비감소.
Objective To determine the mechanism of inhibitory effect of botulinum toxin type A (BTX-A)on parotid gland secretion.Methods Female Wistar rats(n=18)were randomly divided into saline injection group(n=6)and BTX-A injection group(n=12),respectively.0.1 ml of saline was injected into left parotid gland and 2.5 U of BTX-A injected into right parotid gland.Rats were sacrificed at day 7,12 and 35 post-injections respectively for morphology and vasoactive intestinal polypeptide(VIP)immunoreactivity of parotid gland.Results Following BTX-A injection,some atrophic cells and reduction of number of VIP-immunoreactive(VIP-IR)fibers were found in gland and tube at day 7(P＜0.05);at day 12,there was more obvious reduction of VIP-IR fibers around tube and vessels and atrophy of cells in BTX-A injection gland than saline injection gland(P＜0.001);at day 35,the glandular cells and VIP-IR fibers were similar to saline injection group.Conclusions BTX-A is effective for temporary elimination of hyperfunctioning sialorrhea via inhibition of VIP release which plays a key role in modulation of parotid glands secretion.
