CAJ | 학술논문

目的观察辐射及多药耐药基因逆转剂反义寡核苷酸( mdr1ASON)联合5-氟尿嘧啶磁性白蛋白微球(5-Fu-MAMS)靶向治疗耐药裸鼠人胰腺癌模型的效果.方法构建人胰腺癌耐药细胞株(SW1990/Fu),建立裸鼠人胰腺癌耐药模型,选择适当的磁场,施加于肿瘤表面,瘤内注射mdr1ASON及5-Fu-MAMS,观察肿瘤生长,检测其对耐药裸鼠人胰腺癌的治疗效果.结果在外加磁场的磁导向作用下,5-Fu-MAMS能定位于肿瘤组织,联合mdr1ASON能显著抑制肿瘤生长,肿瘤体积抑制率达到85.00％,肿瘤重量抑制率达到87.74％,与其他组比较差异有统计学意义(P＜0.01).结论　辐射促转染的mdr1ASON联合磁性载药微球对肿瘤细胞具有较好的耐药逆转作用.
목적 관찰복사급다약내약기인역전제반의과핵감산( mdr1ASON)연합5-불뇨밀정자성백단백미구(5-Fu-MAMS)파향치료내약라서인이선암모형적효과.방법 구건인이선암내약세포주(SW1990/Fu),건립라서인이선암내약모형,선택괄당적자장,시가우종류표면,류내주사mdr1ASON급5-Fu-MAMS,관찰종류생장,검측기대내약라서인이선암적치료효과.결과 재외가자장적자도향작용하,5-Fu-MAMS능정위우종류조직,연합mdr1ASON능현저억제종류생장,종류체적억제솔체도85.00％,종류중량억제솔체도87.74％,여기타조비교차이유통계학의의(P＜0.01).결론　복사촉전염적mdr1ASON연합자성재약미구대종류세포구유교호적내약역전작용.
Objective To investigate the reversal the multidrug resisitance ( MDR) by MDR gene mdrl antisense oligodeoxynucletdes (ASON) and 5-fluorouracil magnetic albumin miscrospheres (5-FuMAMS) magnetic targeted therapy the human pancreatic cancer in nude mouse model.Methods To construct SW1990/Fu and nude mice experimental model,to investigate the targeted therapy effects of mdr1ASON and 5-Fu-MAMS.Results 5-Fu-MAMS magnetic response was good,its could be well localized in the tumor tissue and markedly inhibit the growth of tumor.The inhibitory rate of tumor size was 85.00％,and that of tumor weight 87.74％.which can effectively degrade proliferating index and accelerate cell death of tumor cell.There were significant differences as compared with other groups( P ＜0.01 ).Conclusion Mdr1 ASON and 5-Fu-MAMS had outstanding reverse effect drug fast on human pancreatic carcinoma model in nude mice.