CAJ | 학술논문

目的探讨异莲心碱(IL)对苯肾上腺素(Phen)诱导猪冠脉血管平滑肌细胞(CASMCs)增殖作用的影响及其机制.方法改良MTT比色法、免疫组织细胞化学技术和Western Blotting等方法.结果 Phen(0.1 μmol·L-1)明显诱导CASMCs增殖,生长因子、原癌基因与hsp70蛋白表达增多.IL(0.03-3 μmol·L-1)浓度依赖性地抑制Phen促猪CASMCs增殖作用.IL(0.1 μmol·L-1)可抑制Phen诱导的PDGF-β和bFGF的蛋白表达增多,并可抑制c-fos, c-myc和hsp70蛋白表达增多.结论 IL具有抗Phen诱导猪CASMCs增殖的作用,其抗增殖机制与抑制生长因子PDGF-β,bFGF及原癌基因c-fos,c-myc和hsp70的增强表达有关.
목적탐토이연심감(IL)대분신상선소(Phen)유도저관맥혈관평활기세포(CASMCs)증식작용적영향급기궤제.방법개량MTT비색법、면역조직세포화학기술화Western Blotting등방법.결과 Phen(0.1 μmol·L-1)명현유도CASMCs증식,생장인자、원암기인여hsp70단백표체증다.IL(0.03-3 μmol·L-1)농도의뢰성지억제Phen촉저CASMCs증식작용.IL(0.1 μmol·L-1)가억제Phen유도적PDGF-β화bFGF적단백표체증다,병가억제c-fos, c-myc화hsp70단백표체증다.결론 IL구유항Phen유도저CASMCs증식적작용,기항증식궤제여억제생장인자PDGF-β,bFGF급원암기인c-fos,c-myc화hsp70적증강표체유관.
Aim To investigate the inhibitory effects and mechanism of action of isoliensinine (IL) on the proliferation of porcine coronary arterial smooth muscle cells (CASMCs) induced by phenylephrine (Phen) and its mechanisms of action. Methods MTT assay, immunohistochemical method and Western blotting were adopted. Results IL (0.03-3 μmol·L-1) could inhibit the CASMCs proliferation induced by Phen (0.1 μmol·L-1) in a concentration-dependent manner. IL (0.1 μmol·L-1) antagonized Phen-induced overexpression of PDGF-β and bFGF from 0.545±0.026 and 0.47±0.03 to 0.458±0.019 and 0.376±0.017 (P<0.01, P<0.01). IL (0.1 μmol·L-1) also decreased c-fos, c-myc and hsp70 overexpression induced by Phen from 0.57±0.04, 0.44±0.04 and (173±36)% to 0.46±0.05, 0.372±0.021 and (115±35)% respectively (P<0.01, P<0.01, P<0.01). Conclusion IL exerted antiproliferative effect on CASMCs induced by phenylephrine, and its mechanisms were related to decrease the overexpression of growth factors (PDGF-β, bFGF), protooncogene (c-fos, c-myc) and hsp70.