CAJ | 학술논문

背景:目前尚缺乏满意的治疗脑梗死的手段.亚低温治疗脑梗死虽然有效,但副作用较大.局部亚低温对脑梗死的治疗可能有较好的作用.目的:观察局部亚低温对大鼠缺血脑组织的保护作用,进一步探讨其作用机制.设计:以实验动物为研究对象,随机对照的基础研究.单位:一所大学医院的神经内科研究所.材料:实验于2000-04/2002-01在哈尔滨医科大学第一临床医学院神经内科动物实验室完成,动物为雄性Wistar大鼠50只,体质量(250±25)g,清洁级.干预:50只大鼠中随机取10只,分为正常组和假手术组,每组5只.余40只随机分为常温脑缺血组和局部亚低温脑缺血组,各20只.制作大鼠大脑中动脉脑缺血模型,进行局部亚低温处理.主要观察指标:各组大鼠脑梗死灶体积、神经功能和血清神经元特异性烯醇化酶的影响.结果:大鼠栓塞48 h时亚低温组和常温组大鼠脑梗死体积分别为(128.95±13.42)和(84.90±11.36)mm3,神经功能评分分别为1.60±0.24和0.95±0.17,血清神经元特异性烯醇化酶浓度分别为(13.55±4.07)和(9.19±3.42)μg/L,差异均有显著性意义.结论:局部亚低温治疗对缺血脑神经元具有保护作用.
배경:목전상결핍만의적치료뇌경사적수단.아저온치료뇌경사수연유효,단부작용교대.국부아저온대뇌경사적치료가능유교호적작용.목적:관찰국부아저온대대서결혈뇌조직적보호작용,진일보탐토기작용궤제.설계:이실험동물위연구대상,수궤대조적기출연구.단위:일소대학의원적신경내과연구소.재료:실험우2000-04/2002-01재합이빈의과대학제일림상의학원신경내과동물실험실완성,동물위웅성Wistar대서50지,체질량(250±25)g,청길급.간예:50지대서중수궤취10지,분위정상조화가수술조,매조5지.여40지수궤분위상온뇌결혈조화국부아저온뇌결혈조,각20지.제작대서대뇌중동맥뇌결혈모형,진행국부아저온처리.주요관찰지표:각조대서뇌경사조체적、신경공능화혈청신경원특이성희순화매적영향.결과:대서전새48 h시아저온조화상온조대서뇌경사체적분별위(128.95±13.42)화(84.90±11.36)mm3,신경공능평분분별위1.60±0.24화0.95±0.17,혈청신경원특이성희순화매농도분별위(13.55±4.07)화(9.19±3.42)μg/L,차이균유현저성의의.결론:국부아저온치료대결혈뇌신경원구유보호작용.
BACKGROUND: There is still no satisfactory therapy for thetreatment of cerebral infarction at present. Although sub-hypothermia is effective in the treatment of cerebral infarction, its side effect is relatively more. Local sub-hypothermia might have favorable effects in the treatment of cerebral infarction.OBJECTIVE: To observe the protective effects of local sub-hypothermia on the ischemic brain tissues in rats to further explore its mechanism.DESIGN: A randomized controlled basic study based on the experimental animals.SETTING: Institute of neurology of a university hospital.MATERIALS: The study was conducted in the Animal Laboratory of Neurology, the First College for Clinical Medicine, Harbin Medical University between April 2000 and January 2002. Fifty male Wistar rats(cleanness grade) with a bodyweight of(250 ± 25) g were selected.INTERVENTIONS: Ten rats were randomly selected from the 50 rats and divided into normal group and sham-operation group with 5 rats each. The rest 40 rats were randomly divided into normal temperature cerebral ischemia group and local hypothermia cerebral ischemia group with 20 rats each. Rat cerebral middle-sized arterial ischemia model was established for local sub-hypothermia disposal.MAIN OUTCOME MEASURES: Impacts on cerebral infarction volume,nerve function, and neuron-specific enolase(NSE) of rats in each group.RESULTS: The cerebral infarction volume at 48 hours after embolism in rats was ( 128.95 ± 13.42) or (84.90 ± 11.36) mm3 respectively, nerve function evaluation was( 1.60 ± 0. 24) or (0. 95 ± 0. 17), and serous NSE concentration was(13.55±4.07) or(9.19±3.42) μg/L in either sub-hypothermia group or normal temperature group, which were significantly different from each other.CONCLUSION: Local sub-hypothermia therapy has protective effects on ischemic cerebral neurons.